[HTML][HTML] Evaluation of Mycobacterium tuberculosis enrichment in metagenomic samples using ONT adaptive sequencing and amplicon sequencing for identification …
…, KKG Tam, KSS Leung, TW Lam, AWS Leung… - Scientific reports, 2023 - nature.com
Sensitive detection of Mycobacterium tuberculosis (TB) in small percentages in metagenomic
samples is essential for microbial classification and drug resistance prediction. However, …
samples is essential for microbial classification and drug resistance prediction. However, …
[HTML][HTML] Low dose inocula of SARS-CoV-2 Alpha variant transmits more efficiently than earlier variants in hamsters
…, RCY Tam, CJ Cremin, TTL Ng, JSL Leung… - Communications …, 2021 - nature.com
Emerging variants of SARS-CoV-2 have been shown to rapidly replace original circulating
strains in humans soon after they emerged. There is a lack of experimental evidence to …
strains in humans soon after they emerged. There is a lack of experimental evidence to …
Probable airborne transmission of Burkholderia pseudomallei causing an urban outbreak of melioidosis during typhoon season in Hong Kong, China
…, SKY Fung, SCY Wong, ICF Ng, TTL Ng… - Emerging Microbes & …, 2023 - Taylor & Francis
Between January 2015 and October 2022, 38 patients with culture-confirmed melioidosis
were identified in the Kowloon West (KW) Region, Hong Kong. Notably, 30 of them were …
were identified in the Kowloon West (KW) Region, Hong Kong. Notably, 30 of them were …
Targeted-Sequencing Workflows for Comprehensive Drug Resistance Profiling of Mycobacterium tuberculosis Cultures Using Two Commercial Sequencing …
K Tafess, TTL Ng, HY Lao, KSS Leung… - Clinical …, 2020 - academic.oup.com
Background The emergence of Mycobacterium tuberculosis with complex drug resistance
profiles necessitates a rapid and comprehensive drug susceptibility test for guidance of patient …
profiles necessitates a rapid and comprehensive drug susceptibility test for guidance of patient …
The Clinical Utility of Two High-Throughput 16S rRNA Gene Sequencing Workflows for Taxonomic Assignment of Unidentifiable Bacterial Pathogens in Matrix …
…, CTM Chan, SHC Jim, JSL Leung… - Journal of Clinical …, 2022 - Am Soc Microbiol
Bacterial pathogens that cannot be identified using matrix-assisted laser desorption
ionization–time of flight mass spectrometry (MALDI-TOF MS) are occasionally encountered in …
ionization–time of flight mass spectrometry (MALDI-TOF MS) are occasionally encountered in …
Long-read sequencing with hierarchical clustering for antiretroviral resistance profiling of mixed human immunodeficiency virus quasispecies
…, HY Lao, WW Lui, CTM Chan, AWS Leung… - Clinical …, 2023 - academic.oup.com
… Timothy Ting-Leung Ng and Junhao Su contributed equally to this work. … For Illumina
sequencing, 1 ng of amplicon was used for library preparation with the Nextera XT DNA Library …
sequencing, 1 ng of amplicon was used for library preparation with the Nextera XT DNA Library …
Nanopore sequencing reveals novel targets for detection and surveillance of human and avian influenza A viruses
…, KH Leung, RWS Poon, ACK Ng… - Journal of Clinical …, 2020 - Am Soc Microbiol
Accurate detection of influenza A virus (IAV) is crucial for patient management, infection
control, and epidemiological surveillance. The World Health Organization and the Centers for …
control, and epidemiological surveillance. The World Health Organization and the Centers for …
[HTML][HTML] Clinical utility of target amplicon sequencing test for rapid diagnosis of drug-resistant Mycobacterium tuberculosis from respiratory specimens
KSS Leung, KKG Tam, TTL Ng, HY Lao… - Frontiers in …, 2022 - frontiersin.org
An in-house-developed target amplicon sequencing by next-generation sequencing
technology (TB-NGS) enables simultaneous detection of resistance-related mutations in …
technology (TB-NGS) enables simultaneous detection of resistance-related mutations in …
[HTML][HTML] Identification of microbial community in the urban environment: The concordance between conventional culture and nanopore 16S rRNA sequencing
…, JSL Leung, LK Lee, ITF Wong, TTL Ng… - Frontiers in …, 2023 - frontiersin.org
Introduction Microbes in the built environment have been implicated as a source of infectious
diseases. Bacterial culture is the standard method for assessing the risk of exposure to …
diseases. Bacterial culture is the standard method for assessing the risk of exposure to …
[HTML][HTML] The clinical utility of Nanopore 16S rRNA gene sequencing for direct bacterial identification in normally sterile body fluids
…, CTM Chan, HWH Lo, MCY Yau, ECM Leung… - Frontiers in …, 2024 - frontiersin.org
The prolonged incubation period of traditional culture methods leads to a delay in diagnosing
invasive infections. Nanopore 16S rRNA gene sequencing (Nanopore 16S) offers a …
invasive infections. Nanopore 16S rRNA gene sequencing (Nanopore 16S) offers a …