To understand regulatory systems, it would be useful to uniformly determine how different
components contribute to the expression of all other genes. We therefore monitored mRNA …

Packaging of DNA into chromatin has a profound impact on gene expression. To understand
how changes in chromatin influence transcription, we analyzed 165 mutants of chromatin …

The transcription cycle can be roughly divided into three stages: initiation, elongation, and
termination. Understanding the molecular events that regulate all these stages requires a …

Transcription factors show rapid and reversible binding to chromatin in living cells, and
transcription occurs in sporadic bursts, but how these phenomena are related is unknown. Using …

To understand relationships between phosphorylation-based signaling pathways, we analyzed
150 deletion mutants of protein kinases and phosphatases in S. cerevisiae using DNA …

Eukaryotic transcription is pervasive, and many of the resulting RNAs are non-coding. It is
unknown whether ubiquitous transcription is functional or simply reflects stochastic …

Growth condition perturbation or gene function disruption are commonly used strategies to
study cellular systems. Although it is widely appreciated that such experiments may involve …

The EKC/KEOPS complex is universally conserved in Archaea and Eukarya and has been
implicated in several cellular processes, including transcription, telomere homeostasis and …

Transcriptional rates are often estimated by fitting the distribution of mature mRNA numbers
measured using smFISH (single molecule fluorescence in situ hybridization) with the …

How distal regulatory elements control gene transcription and chromatin topology is not
clearly defined, yet these processes are closely linked in lineage specification during …