SIMToolbox: a MATLAB toolbox for structured illumination fluorescence microscopy
SIMToolbox is an open-source, modular set of functions for MATLAB equipped with a user-friendly
graphical interface and designed for processing two-dimensional and three-…
graphical interface and designed for processing two-dimensional and three-…
Three-dimensional super-resolution structured illumination microscopy with maximum a posteriori probability image estimation
We introduce and demonstrate a new high performance image reconstruction method for
super-resolution structured illumination microscopy based on maximum a posteriori probability …
super-resolution structured illumination microscopy based on maximum a posteriori probability …
[HTML][HTML] Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging
T Lukeš, D Glatzová, Z Kvíčalová, F Levet… - Nature …, 2017 - nature.com
Quantitative approaches for characterizing molecular organization of cell membrane molecules
under physiological and pathological conditions profit from recently developed super-…
under physiological and pathological conditions profit from recently developed super-…
Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software
With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-molecule
localization microscopy (SMLM), a large set of different data analysis packages have …
localization microscopy (SMLM), a large set of different data analysis packages have …
[HTML][HTML] Correcting for photodestruction in super-resolution optical fluctuation imaging
Super-resolution optical fluctuation imaging overcomes the diffraction limit by analyzing
fluctuations in the fluorophore emission. A key assumption of the imaging is that the fluorophores …
fluctuations in the fluorophore emission. A key assumption of the imaging is that the fluorophores …
[HTML][HTML] Open-source microscope add-on for structured illumination microscopy
MTM Hannebelle, E Raeth, SM Leitao, T Lukeš… - Nature …, 2024 - nature.com
Super-resolution techniques expand the abilities of researchers who have the knowledge
and resources to either build or purchase a system. This excludes the part of the research …
and resources to either build or purchase a system. This excludes the part of the research …
Imaging tissues and cells beyond the diffraction limit with structured illumination microscopy and Bayesian image reconstruction
J Pospíšil, T Lukeš, J Bendesky, K Fliegel… - …, 2019 - academic.oup.com
Background Structured illumination microscopy (SIM) is a family of methods in optical fluorescence
microscopy that can achieve both optical sectioning and super-resolution effects. SIM …
microscopy that can achieve both optical sectioning and super-resolution effects. SIM …
[HTML][HTML] SOFI simulation tool: a software package for simulating and testing super-resolution optical fluctuation imaging
A Girsault, T Lukes, A Sharipov, S Geissbuehler… - PLoS …, 2016 - journals.plos.org
Super-resolution optical fluctuation imaging (SOFI) allows one to perform sub-diffraction
fluorescence microscopy of living cells. By analyzing the acquired image sequence with an …
fluorescence microscopy of living cells. By analyzing the acquired image sequence with an …
[HTML][HTML] Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
H Deschout, T Lukes, A Sharipov, D Szlag… - Nature …, 2016 - nature.com
Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which
remains a challenge for super-resolution microscopy. Here we address this important issue by …
remains a challenge for super-resolution microscopy. Here we address this important issue by …
Self-blinking dyes unlock high-order and multiplane super-resolution optical fluctuation imaging
Most diffraction-unlimited super-resolution imaging critically depends on the switching of
fluorophores between at least two states, often induced using intense laser light and …
fluorophores between at least two states, often induced using intense laser light and …