[HTML][HTML] R-loops at microRNA encoding loci promote co-transcriptional processing of pri-miRNAs in plants

L Gonzalo, I Tossolini, T Gulanicz, DA Cambiagno… - Nature Plants, 2022 - nature.com
In most organisms, the maturation of nascent RNAs is coupled to transcription. Unlike in
animals, the RNA polymerase II (RNAPII) transcribes microRNA genes (MIRNAs) as long and …

mRNA adenosine methylase (MTA) deposits m6A on pri-miRNAs to modulate miRNA biogenesis in Arabidopsis thaliana

SS Bhat, D Bielewicz, T Gulanicz… - Proceedings of the …, 2020 - National Acad Sciences
In Arabidopsis thaliana, the METTL3 homolog, mRNA adenosine methylase (MTA) introduces
N 6 -methyladenosine (m 6 A) into various coding and noncoding RNAs of the plant …

Chromatin-associated microprocessor assembly is regulated by the U1 snRNP auxiliary protein PRP40

A Stepien, J Dolata, T Gulanicz, D Bielewicz… - The Plant …, 2022 - academic.oup.com
In plants, microRNA (miRNA) biogenesis involves cotranscriptional processing of RNA
polymerase II (RNAPII)-generated primary transcripts by a multi-protein complex termed the …

Hyponastic Leaves 1 interacts with RNA Pol II to ensure proper transcription of microRNA genes

…, J Dolata, M Bajczyk, L Szewc, T Gulanicz… - Plant and Cell …, 2023 - academic.oup.com
Hyponastic Leaves 1 (HYL1) [also known as Double-stranded RNA-Binding protein 1 (DRB1)]
is a double-stranded RNA-binding protein involved in microRNA (miRNA) processing in …

Life on the edge: survival and behavioural responses of freshwater gill‐breathing snails to declining water level and substratum drying

M Poznańska, T Kakareko, T Gulanicz… - Freshwater …, 2015 - Wiley Online Library
Reductions in water level and temporary exposure of substratum to air are two of the most
important factors structuring communities in temperate near‐shore zones. Snails are suitable …

[HTML][HTML] Effect of substratum drying on the survival and migrations of a freshwater pulmonate snail Planorbarius corneus (Linnaeus, 1758)

M Poznańska, D Goleniewska, T Gulanicz, T Kakareko… - Hydrobiologia, 2015 - Springer
Pulmonate snails inhabiting the near-shore zone of water bodies experience air exposure
during water level fluctuations and some of them are known to survive long time in temporary …

Effects of water level fluctuations and substratum drying on the survival and behaviour of the invasive freshwater snail Physa acuta Draparnaud, 1805

T Gulanicz, J Kobak… - Marine and Freshwater …, 2018 - CSIRO Publishing
Pulmonate snails live in nearshore zones of waterbodies, experiencing water level fluctuations.
In the present study we investigated the resistance of Physa acuta to desiccation and its …

[HTML][HTML] Fluorescence in situ Localization of Pri-miRNAs in Isolated Arabidopsis thaliana Nuclei

T Gulanicz, A Zienkiewicz, K Zienkiewicz… - Bio …, 2023 - ncbi.nlm.nih.gov
Here, we present an approach combining fluorescence in situ hybridization (FISH) and
immunolabeling for localization of pri-miRNAs in isolated nuclei of A. thaliana. The presented …

DRB1 as a mediator between transcription and microRNA processing

D Bielewicz, J Dolata, M Bajczyk, L Szewc, T Gulanicz… - bioRxiv, 2019 - biorxiv.org
DRB1 (HYL1) is a double-stranded RNA binding protein involved in miRNA processing in
plants. It is a core component of the Microprocessor complex and enhances the efficiency and …

Chromatin-associated microprocessor assembly is regulated by PRP40, the U1 snRNP auxiliary protein

A Stepien, J Dolata, T Gulanicz, D Bielewicz, M Bajczyk… - bioRxiv, 2022 - biorxiv.org
Cotranscriptional processing of RNA polymerase II-generated primary transcripts is a well-documented
phenomenon. We recently showed that in plants, miRNA biogenesis is also a …