Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives

S Noda, T Shirai, S Oyama, A Kondo - Metabolic engineering, 2016 - Elsevier
A synthetic metabolic pathway suitable for the production of chorismate derivatives was
designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to …

[HTML][HTML] Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium

T Osanai, T Shirai, H Iijima, Y Nakaya… - Frontiers in …, 2015 - frontiersin.org
Succinate is a building block compound that the US Department of Energy (DOE) has declared
as important in biorefineries, and it is widely used as a commodity chemical. Here, we …

[HTML][HTML] Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis

T Shirai, K Fujimura, C Furusawa, K Nagahisa… - Microbial cell …, 2007 - Springer
Background Corynebacterium glutamicum has several anaplerotic pathways (anaplerosis),
which are essential for the productions of amino acids, such as lysine and glutamate. It is still …

[HTML][HTML] Organosolv pretreatment of sorghum bagasse using a low concentration of hydrophobic solvents such as 1-butanol or 1-pentanol

…, T Oshima, F Matsuda, M Okamoto, T Shirai… - Biotechnology for …, 2016 - Springer
Background The primary components of lignocellulosic biomass such as sorghum bagasse
are cellulose, hemicellulose, and lignin. Each component can be utilized as a sustainable …

[HTML][HTML] Direct 1,3-butadiene biosynthesis in Escherichia coli via a tailored ferulic acid decarboxylase mutant

Y Mori, S Noda, T Shirai, A Kondo - Nature communications, 2021 - nature.com
The C4 unsaturated compound 1,3-butadiene is an important monomer in synthetic rubber
and engineering plastic production. However, microorganisms cannot directly produce 1,3-…

[HTML][HTML] Engineering a synthetic pathway for maleate in Escherichia coli

S Noda, T Shirai, Y Mori, S Oyama, A Kondo - Nature communications, 2017 - nature.com
Maleate is one of the most important dicarboxylic acids and is used to produce various
polymer compounds and pharmaceuticals. Herein, microbial production of maleate is …

Capillary electrophoresis–mass spectrometry reveals the distribution of carbon metabolites during nitrogen starvation in Synechocystis sp. PCC 6803

T Osanai, A Oikawa, T Shirai… - Environmental …, 2014 - Wiley Online Library
Nitrogen availability is one of the most important factors for the survival of cyanobacteria.
Previous studies on S ynechocystis revealed a contradictory situation with regard to metabolism …

[HTML][HTML] Metabolic engineering design to enhance (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis based on flux balance analysis

N Vikromvarasiri, T Shirai, A Kondo - Microbial cell factories, 2021 - Springer
Background Glycerol is a desirable alternative substrate for 2,3-butanediol (2,3-BD) production
for sustainable development in biotechnological industries and non-food competitive …

[HTML][HTML] Reconstruction of metabolic pathway for isobutanol production in Escherichia coli

…, Y Mori, S Oyama, A Kondo, M Araki, T Shirai - Microbial cell …, 2019 - Springer
Background The microbial production of useful fuels and chemicals has been widely studied.
In several cases, glucose is used as the raw material, and almost all microbes adopt the …

Comparative study of flux redistribution of metabolic pathway in glutamate production by two coryneform bacteria

T Shirai, A Nakato, N Izutani, K Nagahisa, S Shioya… - Metabolic …, 2005 - Elsevier
In amino acid production by coryneform bacteria, study on relationship between change in
enzyme activities and production of a target amino acid is important. In glutamate production, …