Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives
S Noda, T Shirai, S Oyama, A Kondo - Metabolic engineering, 2016 - Elsevier
A synthetic metabolic pathway suitable for the production of chorismate derivatives was
designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to …
designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to …
[HTML][HTML] Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium
T Osanai, T Shirai, H Iijima, Y Nakaya… - Frontiers in …, 2015 - frontiersin.org
Succinate is a building block compound that the US Department of Energy (DOE) has declared
as important in biorefineries, and it is widely used as a commodity chemical. Here, we …
as important in biorefineries, and it is widely used as a commodity chemical. Here, we …
[HTML][HTML] Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis
T Shirai, K Fujimura, C Furusawa, K Nagahisa… - Microbial cell …, 2007 - Springer
Background Corynebacterium glutamicum has several anaplerotic pathways (anaplerosis),
which are essential for the productions of amino acids, such as lysine and glutamate. It is still …
which are essential for the productions of amino acids, such as lysine and glutamate. It is still …
[HTML][HTML] Organosolv pretreatment of sorghum bagasse using a low concentration of hydrophobic solvents such as 1-butanol or 1-pentanol
…, T Oshima, F Matsuda, M Okamoto, T Shirai… - Biotechnology for …, 2016 - Springer
Background The primary components of lignocellulosic biomass such as sorghum bagasse
are cellulose, hemicellulose, and lignin. Each component can be utilized as a sustainable …
are cellulose, hemicellulose, and lignin. Each component can be utilized as a sustainable …
[HTML][HTML] Direct 1,3-butadiene biosynthesis in Escherichia coli via a tailored ferulic acid decarboxylase mutant
Y Mori, S Noda, T Shirai, A Kondo - Nature communications, 2021 - nature.com
The C4 unsaturated compound 1,3-butadiene is an important monomer in synthetic rubber
and engineering plastic production. However, microorganisms cannot directly produce 1,3-…
and engineering plastic production. However, microorganisms cannot directly produce 1,3-…
[HTML][HTML] Engineering a synthetic pathway for maleate in Escherichia coli
S Noda, T Shirai, Y Mori, S Oyama, A Kondo - Nature communications, 2017 - nature.com
Maleate is one of the most important dicarboxylic acids and is used to produce various
polymer compounds and pharmaceuticals. Herein, microbial production of maleate is …
polymer compounds and pharmaceuticals. Herein, microbial production of maleate is …
Capillary electrophoresis–mass spectrometry reveals the distribution of carbon metabolites during nitrogen starvation in Synechocystis sp. PCC 6803
T Osanai, A Oikawa, T Shirai… - Environmental …, 2014 - Wiley Online Library
Nitrogen availability is one of the most important factors for the survival of cyanobacteria.
Previous studies on S ynechocystis revealed a contradictory situation with regard to metabolism …
Previous studies on S ynechocystis revealed a contradictory situation with regard to metabolism …
[HTML][HTML] Metabolic engineering design to enhance (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis based on flux balance analysis
N Vikromvarasiri, T Shirai, A Kondo - Microbial cell factories, 2021 - Springer
Background Glycerol is a desirable alternative substrate for 2,3-butanediol (2,3-BD) production
for sustainable development in biotechnological industries and non-food competitive …
for sustainable development in biotechnological industries and non-food competitive …
[HTML][HTML] Reconstruction of metabolic pathway for isobutanol production in Escherichia coli
Background The microbial production of useful fuels and chemicals has been widely studied.
In several cases, glucose is used as the raw material, and almost all microbes adopt the …
In several cases, glucose is used as the raw material, and almost all microbes adopt the …
Comparative study of flux redistribution of metabolic pathway in glutamate production by two coryneform bacteria
T Shirai, A Nakato, N Izutani, K Nagahisa, S Shioya… - Metabolic …, 2005 - Elsevier
In amino acid production by coryneform bacteria, study on relationship between change in
enzyme activities and production of a target amino acid is important. In glutamate production, …
enzyme activities and production of a target amino acid is important. In glutamate production, …