To reduce the number of replicas required in the conventional replica exchange method for
huge systems, recently the replica exchange with solute tempering (REST) method was …

Molecular evolution based on mutagenesis is widely used in protein engineering. However,
optimal proteins are often difficult to obtain due to a large sequence space. Here, we …

Early in vivo studies demonstrated the involvement of a tumor-suppressing transcription
factor, p53, into cellular droplets such as Cajal and promyelocytic leukemia protein bodies, …

The poor aqueous solubility of drug substances hampers their broader applications. This paper
describes a de novo strategy to increase the aqueous solubility of drug substances using …

Machine learning (ML) is becoming an attractive tool in mutagenesis-based protein
engineering because of its ability to design a variant library containing proteins with a desired …

Liquid–liquid phase separation (LLPS) of proteins and nucleic acids to form membraneless
cellular compartments is considered to be involved in various biological functions. The RNA-…

Aromatic carbon nanomaterials, such as carbon nanotubes and graphene, undergo protein
adsorption in the early stages of their uptake into biological systems, which determines their …

Motivation Recent studies have revealed that large numbers of non-coding RNAs are
transcribed in humans, but only a few of them have been identified with their functions. …

Non-equilibrium molecular dynamics (NEMD) simulation has been recognized as a
powerful tool for examining biomolecules and provides fruitful insights into not only non-equilibrium …

We herein report the first covalent G-site-binding inhibitor for GST, GS-ESF (1), which
irreversibly inhibited the GSTP1-1 function. LC-MS/MS and X-ray structure analyses of the …