Studying the role of essential proteins is dependent upon a method for rapid inactivation, in
order to study the immediate phenotypic consequences. Auxin-inducible degron (AID) …

The conditional depletion of a protein of interest (POI) is useful not only for loss-of-function
studies, but also for the modulation of biological pathways. Technologies that work at the level …

Centromeres play several important roles in ensuring proper chromosome segregation. Not
only do they promote kinetochore assembly for microtubule attachment, but they also …

Termination is a ubiquitous phase in every transcription cycle but is incompletely
understood and a subject of debate. We used gene editing as a new approach to address its …

The temporal order of DNA replication [replication timing (RT)] is correlated with chromatin
modifications and three-dimensional genome architecture; however, causal links have not …

The cohesin- and condensin-related SMC5/6 complex has largely been studied in the
context of DNA repair. Nevertheless, SMC5/6 has an undefined essential function even in the …

10.7554/eLife.36559.001 To position the mitotic spindle within the cell, dynamic plus ends of
astral microtubules are pulled by membrane-associated cortical force-generating machinery…

Controlling protein expression using a degron is advantageous because the protein of
interest can be rapidly depleted in a reversible manner. We pioneered the development of the …

High-intensity transcription and replication supercoil DNA to levels that can impede or halt
these processes. As a potent transcription amplifier and replication accelerator, the proto-…

Embryonic development is dictated by tight regulation of DNA replication, cell division and
differentiation. Mutations in DNA repair and replication genes disrupt this equilibrium, giving …