The ability to perturb genes in human cells is crucial for elucidating gene function and holds
great potential for finding therapeutic targets for diseases such as cancer. To extend the …

Background Pooled library CRISPR/Cas9 knockout screening across hundreds of cell lines
has identified genes whose disruption leads to fitness defects, a critical step in identifying …

Capitalizing on the inherent multiplexing capability of AsCpf1, we developed a multiplexed,
high-throughput screening strategy that minimizes library size without sacrificing gene …

SLC7A11-mediated cystine uptake suppresses ferroptosis yet promotes cell death under
glucose starvation; the nature of the latter cell death remains unknown. Here we show that …

53BP1 is a chromatin-binding protein that regulates the repair of DNA double-strand breaks
by suppressing the nucleolytic resection of DNA termini 1 , 2 . This function of 53BP1 …

The adaptation of CRISPR/SpCas9 technology to mammalian cell lines is transforming the
study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs) …

The genetic circuits that allow cancer cells to evade destruction by the host immune system
remain poorly understood 1 , 2 – 3 . Here, to identify a phenotypically robust core set of …

Technological advancement has opened the door to systematic genetics in mammalian cells.
Genome‐scale loss‐of‐function screens can assay fitness defects induced by partial gene …

The observation that BRCA1- and BRCA2-deficient cells are sensitive to inhibitors of poly(ADP–ribose)
polymerase (PARP) has spurred the development of cancer therapies that use …

Forward genetic screens with CRISPR–Cas9 genome editing enable high-resolution
detection of genetic vulnerabilities in cancer cells. We conducted genome-wide CRISPR–Cas9 …