Targeted genome editing of bacteria within microbial communities

…, C He, M Xu, Z Zhou, DC Smock, K Tang, TK Owens… - BioRxiv, 2020 - biorxiv.org
K. michiganensis are known to be naturally competent. We conducted a second experiment
to compare the insertion efficiency of K… LOD for natural transformation of K. michiganensis in …

Species-and site-specific genome editing in complex bacterial communities

…, R Rovinsky, DCJ Smock, K Tang, TK Owens… - Nature …, 2022 - nature.com
Understanding microbial gene functions relies on the application of experimental genetics
in cultured microorganisms. However, the vast majority of bacteria and archaea remain …

Selective carbon sources influence the end products of microbial nitrate respiration

…, AE Kazakov, AV Carr, JV Kuehl, TK Owens… - The ISME …, 2020 - academic.oup.com
Respiratory and catabolic genes are differentially distributed across microbial genomes.
Thus, specific carbon sources may favor different respiratory processes. We profiled the …

Deciphering microbial metal toxicity responses via random bar code transposon site sequencing and activity-based metabolomics

…, VV Trotter, X Ge, FL Poole, TK Owens… - Applied and …, 2021 - Am Soc Microbiol
To uncover metal toxicity targets and defense mechanisms of the facultative anaerobe Pantoea
sp. strain MT58 (MT58), we used a multiomic strategy combining two global techniques, …

[HTML][HTML] Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria

VK Mutalik, PS Novichkov, MN Price, TK Owens… - Nature …, 2019 - nature.com
A major challenge in genomics is the knowledge gap between sequence and its encoded
function. Gain-of-function methods based on gene overexpression are attractive avenues for …

Impact of inoculation practices on microbiota assembly and community stability in a fabricated ecosystem

…, PF Andeer, TK Owens, K Zhalnina… - Phytobiomes …, 2024 - Am Phytopath Society
Studying plant–microbe–soil interactions is challenging due to their high complexity and
variability in natural ecosystems. While fabricated ecosystems provide opportunities to …

“Invisible in the Most Tragic of Ways”: Exploring Internalized Transphobia and Coping Through Photovoice

TK Owens, L Mizock, AJ Ormerod… - Health promotion …, 2023 - journals.sagepub.com
Transgender and gender diverse (TGD) individuals experience minority stress that includes
internalizing the negative attitudes that exist in society about those whose identities rest …

Exploring Internalized Transphobia and Coping through Photovoice

TK Owens - 2020 - search.proquest.com
Transgender and gender diverse (TGD) individuals experience minority stress that includes
internalizing the negative attitudes that exist in society about those whose identities rest …

Deciphering Microbial Metal Toxicity Responses using RB-TnSeq and Activity-Based Metabolomics

…, VV Trotter, X Ge, FL Poole, TK Owens… - Applied and …, 2021 - osti.gov
To uncover metal toxicity targets and defense mechanisms of the facultative anaerobe Pantoea
sp. strain MT58 (MT58), we used a multiomic strategy combining two global techniques, …

Dub-seq: dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits

VK Mutalik, PS Novichkov, MN Price, TK Owens… - bioRxiv, 2018 - biorxiv.org
A major challenge in genomics is the knowledge gap between sequence and its encoded
function. Gain-of-function methods based on gene overexpression are attractive avenues for …