Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells

…, I Takayama, N Nagata, T Sekizuka… - Proceedings of the …, 2020 - National Acad Sciences
A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2),
which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently …

Haplotype networks of SARS-CoV-2 infections in the Diamond Princess cruise ship outbreak

T Sekizuka, K Itokawa, T Kageyama… - Proceedings of the …, 2020 - National Acad Sciences
The Diamond Princess cruise ship was put under quarantine offshore Yokohama, Japan,
after a passenger who disembarked in Hong Kong was confirmed as a coronavirus disease …

Identification of Kudoa septempunctata as the Causative Agent of Novel Food Poisoning Outbreaks in Japan by Consumption of Paralichthys olivaceus in Raw Fish

T Kawai, T Sekizuka, Y Yahata, M Kuroda… - Clinical infectious …, 2012 - academic.oup.com
Background. Outbreaks of an unidentified food-borne illness associated with the consumption
of raw fish have increased in Japan since 2003. Those affected with this illness develop …

The genome landscape of the african green monkey kidney-derived vero cell line

…, T Yamaji, N Hirayama, F Kasai, T Sekizuka… - DNA …, 2014 - academic.oup.com
Continuous cell lines that originate from mammalian tissues serve as not only invaluable
tools for life sciences, but also important animal cell substrates for the production of various …

[HTML][HTML] Disentangling primer interactions improves SARS-CoV-2 genome sequencing by multiplex tiling PCR

K Itokawa, T Sekizuka, M Hashino, R Tanaka… - PloS one, 2020 - journals.plos.org
Since December 2019, the coronavirus disease 2019 (COVID-19) caused by a novel
coronavirus SARS-CoV-2 has rapidly spread to almost every nation in the world. Soon after the …

[HTML][HTML] Clinicopathologic and immunohistochemical findings from autopsy of patient with COVID-19, Japan

…, H Katano, M Tobiume, T Sekizuka… - Emerging infectious …, 2020 - ncbi.nlm.nih.gov
An autopsy of a patient in Japan with coronavirus disease indicated pneumonia lung pathology,
manifested as diffuse alveolar damage. We detected severe acute respiratory syndrome …

The Host Protease TMPRSS2 Plays a Major Role in In Vivo Replication of Emerging H7N9 and Seasonal Influenza Viruses

…, M Anraku, M Abe, N Nakajima, T Sekizuka… - Journal of …, 2014 - Am Soc Microbiol
Proteolytic cleavage of the hemagglutinin (HA) protein is essential for influenza A virus (IAV)
to acquire infectivity. This process is mediated by a host cell protease(s) in vivo. The type II …

[HTML][HTML] Characterization of Quasispecies of Pandemic 2009 Influenza A Virus (A/H1N1/2009) by De Novo Sequencing Using a Next-Generation DNA Sequencer

…, N Nakajima, M Tobiume, A Ainai, T Sekizuka… - PloS one, 2010 - journals.plos.org
Pandemic 2009 influenza A virus (A/H1N1/2009) has emerged globally. In this study, we
performed a comprehensive detection of potential pathogens by de novo sequencing using a …

[HTML][HTML] Complete Sequencing of the blaNDM-1-Positive IncA/C Plasmid from Escherichia coli ST38 Isolate Suggests a Possible Origin from Plant Pathogens

T Sekizuka, M Matsui, K Yamane, F Takeuchi… - PloS one, 2011 - journals.plos.org
The complete sequence of the plasmid pNDM-1_Dok01 carrying New Delhi metallo-β-lactamase
(NDM-1) was determined by whole genome shotgun sequencing using Escherichia …

Inhibition of Streptococcus mutans Biofilm Formation by Streptococcus salivarius FruA

…, T Kawarai, N Narisawa, T Sekizuka… - Applied and …, 2011 - Am Soc Microbiol
The oral microbial flora consists of many beneficial species of bacteria that are associated
with a healthy condition and control the progression of oral disease. Cooperative interactions …