Precursor mRNA splicing is one of the most highly regulated processes in metazoan species.
In addition to generating vast repertoires of RNAs and proteins, splicing has a profound …

Background: Histone H3 lysine 9 (H3-K9) methylation and DNA methylation are characteristic
hallmarks of mammalian heterochromatin. H3-K9 methylation was recently shown to be a …

Chromatin is important for the regulation of transcription and other functions, yet the
diversity of chromatin composition and the distribution along chromosomes are still poorly …

Histone lysine methylation has been shown to index silenced chromatin regions at, for
example, pericentric heterochromatin or of the inactive X chromosome. Here, we examined the …

Alternative splicing (AS) of precursor RNAs is responsible for greatly expanding the
regulatory and functional capacity of eukaryotic genomes. Of the different classes of AS, intron …

Alternative splicing (AS) generates remarkable regulatory and proteomic complexity in
metazoans. However, the functions of most AS events are not known, and programs of regulated …

… DIP RNase-sensitivity analysis was carried out by adding 50 U of RNase H (Invitrogen
catalogue number 18021-014) in 10× RNase H buffer (75 mM KCl, 50 mM Tris pH 8.3, 3 mM …

Networks of coordinated alternative splicing (AS) events play critical roles in development
and disease. However, a comprehensive knowledge of the factors that regulate these …

… Subsets were compared using two-tailed Mann–Whitney U-tests. Tests were performed
only between groups with indicated P values. hgRNA guides per group: 3,310 (Cas9 exonic–…

… , and their positional relationship with distal C/U-rich motifs was not detected. Moreover, … In
Vivo To investigate whether nSR100 binds to the UGC, C/U-rich, and/ or other motifs, we used …