The insulin-like growth factor 1 receptor (IGF-1R) plays crucial roles in developmental and
cancer biology. Most of its biological effects have been ascribed to its tyrosine kinase activity, …

… In a second pilot study, eight samples were sequenced in parallel at NGI-U and NGI-S to … ,
we conclude that the site effects between NGI-U and NGI-S are negligible. After variant quality …

Objective To determine whether genetic variants of the interferon regulatory factor 5 (IRF‐5)
and Tyk‐2 genes are associated with rheumatoid arthritis (RA). Methods Five single‐…

… To remove remaining dNTPs and primers, 7.5 µl aliquots of the concentrated PCR‐products
were treated with 0.1 U/µl shrimp alkaline phosphatase and 0.5 U/µl exonuclease I (USB …

Clinical applications of precision oncology require accurate tests that can distinguish true
cancer-specific mutations from errors introduced at each step of next-generation sequencing (…

Background: The renin-angiotensin-aldosterone system (RAAS) is important for the development
of hypertension, and several antihypertensive drugs target this system. Our aim was to …

Screening for gene copy‐number alterations (CNAs) has improved by applying genome‐wide
microarrays, where SNP arrays also allow analysis of loss of heterozygozity (LOH). We …

… Fifty to 500 ng of DNA and 3.5 U of DNA polymerase are standard amounts in 100 μL
multiplex PCR reaction. A touchdown PCR procedure that functions well for us is: Initial activation …

Population structure can provide novel insight into the human past, and recognizing and
correcting for such stratification is a practical concern in gene mapping by many association …

In the microarray format of the minisequencing method multiple oligonucleotide primers
immobilised on a glass surface are extended with fluorescent ddNTPs using a DNA polymerase. …