The actin cytoskeleton is a vital component of several key cellular and developmental
processes in eukaryotes. Many proteins that interact with filamentous and/or monomeric actin …

The actin cytoskeleton plays a fundamental role in various motile and morphogenetic
processes involving membrane dynamics. We show that actin-binding proteins MIM (missing-in-…

Drugs with prolonged on-target residence times often show superior efficacy, yet general
strategies for optimizing drug-target residence time are lacking. Here we made progress toward …

Fragment-based ligand design and covalent targeting of noncatalytic cysteines have been
employed to develop potent and selective kinase inhibitors. Here, we combine these …

Actin dynamics provide the driving force for many cellular processes including motility and
endocytosis. Among the central cytoskeletal regulators are actin-depolymerizing factor (ADF)/…

Covering: up to the end of 2019 Diverse natural product small molecules have allowed
critical insights into processes that govern eukaryotic cells' ability to secrete cytosolically …

Mycolactone, an immunosuppressive macrolide released by the human pathogen Mycobacterium
ulcerans, was previously shown to impair Sec61-dependent protein translocation, but …

Apratoxin A is a cytotoxic natural product that prevents the biogenesis of secretory and
membrane proteins. Biochemically, apratoxin A inhibits cotranslational translocation into the ER, …

Rapamycin and FK506 are macrocyclic natural products with an extraordinary mode of action,
in which they form binary complexes with FK506-binding protein (FKBP) through a shared …

10.7554/eLife.01483.001 Membrane protein biogenesis requires the coordinated movement
of hydrophobic transmembrane domains (TMD) from the cytosolic vestibule of the Sec61 …