User profiles for Vladimir Besada
 | vladimir besadacentro de ingeniería genética y biotecnología Verified email at cigb.edu.cu Cited by 2628 |
Purification and characterization of two hemolysins from Stichodactyla helianthus
Two hemolysins, Sticholysin I (St I) and Sticholysin II (St II) were purified from the sea anemone
Stichodactyla helianthus combining gel filtration and ion exchange chromatography. The …
Stichodactyla helianthus combining gel filtration and ion exchange chromatography. The …
Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris
…, J Valdés, Y Terrero, A Hidalgo, L Plana, V Besada… - Biotechnology …, 2005 - Springer
We have found a direct relationship between protein production in Pichia pastoris and the
number of introduced synthetic genes of miniproinsulin (MPI), fused to the Saccharomyces …
number of introduced synthetic genes of miniproinsulin (MPI), fused to the Saccharomyces …
CIGB-300, a novel proapoptotic peptide that impairs the CK2 phosphorylation and exhibits anticancer properties both in vitro and in vivo
Protein Kinase CK2 is a serine-threonine kinase frequently deregulated in many human
tumors. Here, we hypothesized that a peptide binder to the CK2 phosphoacceptor site could …
tumors. Here, we hypothesized that a peptide binder to the CK2 phosphoacceptor site could …
A method for determination of N-glycosylation sites in glycoproteins by collision-induced dissociation analysis in fast atom bombardment mass spectrometry …
Previously, a combined use of fast atom bombardment (FAB) mass spectrometry and peptide
N-glycosidase F, an enzyme that cleaves the β-aspartylglycosylamine linkage of Asn-…
N-glycosidase F, an enzyme that cleaves the β-aspartylglycosylamine linkage of Asn-…
Differentiating α‐and β‐aspartic acids by electrospray ionization and low‐energy tandem mass spectrometry
…, Y Satomi, L Betancourt, V Besada… - Rapid …, 2000 - Wiley Online Library
Spectra obtained by low‐energy electrospray ionization tandem mass spectrometry (ESI‐MS/MS)
of 34 peptides containing aspartic acids at position n were studied and unambiguously …
of 34 peptides containing aspartic acids at position n were studied and unambiguously …
[HTML][HTML] Synthesis, LC-MS/MS analysis, and biological evaluation of two vaccine candidates against ticks based on the antigenic P0 peptide from R. sanguineus linked to the …
A peptide from the P0 acidic ribosomal protein (pP0) of ticks conjugated to keyhole limpet
hemocyanin from Megathura crenulata has shown to be effective against different tick species …
hemocyanin from Megathura crenulata has shown to be effective against different tick species …
[PDF][PDF] Recombinant novel pituitary adenylate cyclase-activating polypeptide from African catfish (Clarias gariepinus) authenticates its biological function as a growth …
…, R Morales, O Gonzalez, J Acosta, V Besada… - Journal of …, 2008 - academia.edu
Nowadays, the studies of pituitary adenylate cyclase-activating polypeptide (PACAP)-related
peptide (PRP) and PACAP in non-mammalian vertebrates, especially in fish, have paid …
peptide (PRP) and PACAP in non-mammalian vertebrates, especially in fish, have paid …
Automated interpretation of low‐energy collision‐induced dissociation spectra by SeqMS, a software aid for de novo sequencing by tandem mass spectrometry
…, T Shima, N Okumura, V Besada… - …, 2000 - Wiley Online Library
SeqMS, a software aid for de novo sequencing by tandem mass spectrometry (MS/MS),
which was initially developed for the automated interpretation of high‐energy collision‐induced …
which was initially developed for the automated interpretation of high‐energy collision‐induced …
[HTML][HTML] In-solution buffer-free digestion allows full-sequence coverage and complete characterization of post-translational modifications of the receptor-binding …
…, M Limonta, E Pimentel, M Ayala, V Besada… - Analytical and …, 2021 - Springer
Subunit vaccines based on the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2
provide one of the most promising strategies to fight the COVID-19 pandemic. The …
provide one of the most promising strategies to fight the COVID-19 pandemic. The …
Isoelectric point optimization using peptide descriptors and support vector machines
IPG (Immobilized pH Gradient) based separations are frequently used as the first step in
shotgun proteomics methods; it yields an increase in both the dynamic range and resolution of …
shotgun proteomics methods; it yields an increase in both the dynamic range and resolution of …