User profiles for W. J. Greenleaf
 | William GreenleafAssociate Professor of Genetics, Stanford University Verified email at stanford.edu Cited by 36810 |
Chromatin accessibility and the regulatory epigenome
SL Klemm, Z Shipony, WJ Greenleaf - Nature Reviews Genetics, 2019 - nature.com
Physical access to DNA is a highly dynamic property of chromatin that plays an essential role
in establishing and maintaining cellular identity. The organization of accessible chromatin …
in establishing and maintaining cellular identity. The organization of accessible chromatin …
High-resolution, single-molecule measurements of biomolecular motion
WJ Greenleaf, MT Woodside… - Annu. Rev. Biophys …, 2007 - annualreviews.org
Many biologically important macromolecules undergo motions that are essential to their
function. Biophysical techniques can now resolve the motions of single molecules down to the …
function. Biophysical techniques can now resolve the motions of single molecules down to the …
Single-molecule studies of RNA polymerase: motoring along
Single-molecule techniques have advanced our understanding of transcription by RNA
polymerase (RNAP). A new arsenal of approaches, including single-molecule fluorescence, …
polymerase (RNAP). A new arsenal of approaches, including single-molecule fluorescence, …
Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position
… We thank members of Greenleaf and Chang labs for discussion, A. Burnet and S. Kim lab
and the Stanford flow-cytometry core facility for assistance with FACS sorting, A. …
and the Stanford flow-cytometry core facility for assistance with FACS sorting, A. …
ATAC‐seq: a method for assaying chromatin accessibility genome‐wide
…, B Wu, HY Chang, WJ Greenleaf - Current protocols in …, 2015 - Wiley Online Library
This unit describes Assay for Transposase‐Accessible Chromatin with high‐throughput
sequencing (ATAC‐seq), a method for mapping chromatin accessibility genome‐wide. This …
sequencing (ATAC‐seq), a method for mapping chromatin accessibility genome‐wide. This …
Single-cell chromatin accessibility reveals principles of regulatory variation
… We thank members of Greenleaf and Chang laboratories, as well as the Fluidigm team, …
Here, William Greenleaf and colleagues describe a single-cell transposase-based method, …
Here, William Greenleaf and colleagues describe a single-cell transposase-based method, …
An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues
We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility
profiling that works across multiple applications with substantial improvement of signal-to-…
profiling that works across multiple applications with substantial improvement of signal-to-…
[HTML][HTML] ArchR is a scalable software package for integrative single-cell chromatin accessibility analysis
… We thank members of the Greenleaf and Chang laboratories for helpful comments. This
work was supported by the NIH RM1-HG007735 and UM1-HG009442 (to HYC and WJG), R35-…
work was supported by the NIH RM1-HG007735 and UM1-HG009442 (to HYC and WJG), R35-…
Direct observation of base-pair stepping by RNA polymerase
During transcription, RNA polymerase (RNAP) moves processively along a DNA template,
creating a complementary RNA. Here we present the development of an ultra-stable optical …
creating a complementary RNA. Here we present the development of an ultra-stable optical …
Lineage-specific and single-cell chromatin accessibility charts human hematopoiesis and leukemia evolution
We define the chromatin accessibility and transcriptional landscapes in 13 human primary
blood cell types that span the hematopoietic hierarchy. Exploiting the finding that the …
blood cell types that span the hematopoietic hierarchy. Exploiting the finding that the …