User profiles for W. Backer

Wilfried De Backer

Professor of Respiratory Medicine
Verified email at uantwerpen.be
Cited by 15808

Aqueous two-phase extraction as a platform in the biomanufacturing industry: economical and environmental sustainability

PAJ Rosa, AM Azevedo, S Sommerfeld, W Bäcker… - Biotechnology …, 2011 - Elsevier
… A 25% (w/w) loading of cells supernatant in the bottom phosphate-rich phase from the first
extraction step was considered. All the inlet phases were assumed to be prepared according …

The size effect in metal cutting

WR Backer, ER Marshall… - … of the American …, 1952 - asmedigitalcollection.asme.org
… V ariation op C hip L ength W ith W heel D epthof C ut — M … V ariationof S hear - E nergy W
ith S pecimen S ize ; SAIO … Walker, and W. Littmann of the Metal Cutting Research Laboratory …

Prevalence of sleep disturbances among young adults in three European countries

C Janson, T Gislason, WD Backer, P Plaschke… - Sleep, 1995 - academic.oup.com
The aim of this investigation was to study the geographic variation in sleep complaints and
to identify risk factors for sleep disturbances in three European countries: Iceland (Reykjavik), …

Application of aqueous two-phase systems to antibody purification: a multi-stage approach

…, M Mutter, MR Aires-Barros, W Bäcker - Journal of …, 2009 - Elsevier
… The CHO cells supernatant was added in the end and consisted of 25% (w/w) of the final …
w/w) of the bottom phase. ATPS with different NaCl concentrations, such as 7.5% and 10% (w/w

Continuous purification of antibodies from cell culture supernatant with aqueous two‐phase systems: From concept to process

…, S Sommerfeld, M Mutter, W Bäcker… - Biotechnology …, 2013 - Wiley Online Library
… % w/w PEG 3350 Da, 40% w/w phosphate buffer and solid NaCl. Phosphate buffer at pH 6
was prepared using a NaH 2 PO 4 /K 2 HPO 4 mass ratio of 1.27. The BP (BP extraction) was …

Continuous aqueous two-phase extraction of human antibodies using a packed column

…, AM Azevedo, S Sommerfeld, W Bäcker… - … of Chromatography B, 2012 - Elsevier
… It was also possible to observe that the spiking of the cells supernatant and the increase
of the NaCl concentration in the ATPS from 7.5% (w/w) to 10% (w/w) has apparently no …

Downstream processing of antibodies: single-stage versus multi-stage aqueous two-phase extraction

…, IF Ferreira, S Sommerfeld, W Bäcker… - … of Chromatography A, 2009 - Elsevier
… (w/w) PEG stock solution and was added to the ATPS as an additive at a concentration of
0–2% (w/w). All systems contained 10 mM phosphate buffer and were loaded with 40% (w/w) …

Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into Drosophila flight muscles

J Sauerwald, W Backer, T Matzat, F Schnorrer… - Elife, 2019 - elifesciences.org
10.7554/eLife.48857.001 Tubular networks like the vasculature extend branches throughout
animal bodies, but how developing vessels interact with and invade tissues is not well …

Production of separation-nozzle systems for uranium enrichment by a combination of X-ray lithography and galvanoplastics

EW Backer, W Ehrfeld, D Münchmeyer, H Betz… - …, 1982 - Springer
X-ray lithography using synchrotron radiation has been applied in a multi-step process for
the production of plastic moulds to be used in the fabrication of separation nozzles by …

Acute manipulation and real-time visualization of membrane trafficking and exocytosis in Drosophila

J Glashauser, C Camelo, M Hollmann, W Backer… - Developmental Cell, 2023 - cell.com
Intracellular trafficking of secretory proteins plays key roles in animal development and
physiology, but so far, tools for investigating the dynamics of membrane trafficking have been …