[HTML][HTML] A time-and cost-efficient system for high-level protein production in mammalian cells
AR Aricescu, W Lu, EY Jones - Acta Crystallographica Section D …, 2006 - scripts.iucr.org
Most proteins for structural biology studies are produced by high-level expression in Escherichia
coli. However, prokaryotic based expression systems fail to generate correctly folded …
coli. However, prokaryotic based expression systems fail to generate correctly folded …
Proteoglycan-specific molecular switch for RPTPσ clustering and neuronal extension
…, Y Shen, AP Tenney, C Siebold, GC Sutton, W Lu… - Science, 2011 - science.org
Heparan and chondroitin sulfate proteoglycans (HSPGs and CSPGs, respectively) regulate
numerous cell surface signaling events, with typically opposite effects on cell function. …
numerous cell surface signaling events, with typically opposite effects on cell function. …
Modular mechanism of Wnt signaling inhibition by Wnt inhibitory factor 1
Wnt morphogens control embryonic development and homeostasis in adult tissues. In
vertebrates the N-terminal WIF domain (WIF-1 WD ) of Wnt inhibitory factor 1 (WIF-1) binds Wnt …
vertebrates the N-terminal WIF domain (WIF-1 WD ) of Wnt inhibitory factor 1 (WIF-1) binds Wnt …
Structure of a tyrosine phosphatase adhesive interaction reveals a spacer-clamp mechanism
Cell-cell contacts are fundamental to multicellular organisms and are subject to exquisite
levels of control. Human RPTPμ is a type IIB receptor protein tyrosine phosphatase that both …
levels of control. Human RPTPμ is a type IIB receptor protein tyrosine phosphatase that both …
[HTML][HTML] Structural basis for extracellular cis and trans RPTPσ signal competition in synaptogenesis
Receptor protein tyrosine phosphatase sigma (RPTPσ) regulates neuronal extension and
acts as a presynaptic nexus for multiple protein and proteoglycan interactions during …
acts as a presynaptic nexus for multiple protein and proteoglycan interactions during …
Molecular analysis of receptor protein tyrosine phosphatase μ‐mediated cell adhesion
AR Aricescu, WC Hon, C Siebold, W Lu… - The EMBO …, 2006 - embopress.org
Type IIB receptor protein tyrosine phosphatases (RPTPs) are bi‐functional cell surface
molecules. Their ectodomains mediate stable, homophilic, cell‐adhesive interactions, whereas …
molecules. Their ectodomains mediate stable, homophilic, cell‐adhesive interactions, whereas …
[HTML][HTML] Automation of large scale transient protein expression in mammalian cells
Traditional mammalian expression systems rely on the time-consuming generation of stable
cell lines; this is difficult to accommodate within a modern structural biology pipeline. …
cell lines; this is difficult to accommodate within a modern structural biology pipeline. …
Antiepileptic drug carbamazepine binds to a novel pocket on the Wnt receptor Frizzled-8
Misregulation of Wnt signaling is common in human cancer. The development of small molecule
inhibitors against the Wnt receptor, frizzled (FZD), may have potential in cancer therapy. …
inhibitors against the Wnt receptor, frizzled (FZD), may have potential in cancer therapy. …
Structural characterization of melatonin as an inhibitor of the Wnt deacylase Notum
The hormone melatonin, secreted from the pineal gland, mediates multiple physiological
effects including modulation of Wnt/β‐catenin signalling. The Wnt palmitoleate lipid …
effects including modulation of Wnt/β‐catenin signalling. The Wnt palmitoleate lipid …
Production of cell surface and secreted glycoproteins in mammalian cells
E Seiradake, Y Zhao, W Lu, AR Aricescu… - Structural Proteomics …, 2015 - Springer
Mammalian protein expression systems are becoming increasingly popular for the production
of eukaryotic secreted and cell surface proteins. Here we describe methods to produce …
of eukaryotic secreted and cell surface proteins. Here we describe methods to produce …