We developed a new method for the conditional regulation of CRISPR/Cas9 activity in
mammalian cells and zebrafish embryos using photochemically activated, caged guide RNAs (…

The recently discovered CRISPR/Cas9 endonuclease system, comprised of a guide RNA for
the recognition of a DNA target and the Cas9 nuclease protein for binding and processing …

The type III-E CRISPR–Cas7-11 effector binds a CRISPR RNA (crRNA) and the putative
protease Csx29 and catalyzes crRNA-guided RNA cleavage. We report cryo–electron …

Programmable genome integration of large, diverse DNA cargo without DNA repair of
exposed DNA double-strand breaks remains an unsolved challenge in genome editing. We …

Programmable approaches to sense and respond to the presence of specific RNAs in
biological systems have broad applications in research, diagnostics, and therapeutics. Here we …

The type III-E CRISPR-Cas effector Cas7-11, with dual RNase activities for precursor CRISPR
RNA (pre-crRNA) processing and crRNA-guided target RNA cleavage, is a new platform …

Sargassum fusiforme (Harv.) Setchel, a kind of brown algae, has been applied as a therapeutic
for thousands of years. This study was designed to investigate the antitumor effects of the …

ObjectiveThe aims of the present paper were to assess the nutritional status of children under
5 years old using the 2006 WHO Child Growth Standards (‘the WHO standards’) and to …

The radiation forces and trajectories of Rayleigh dielectric particles induced by one-dimensional
Airy beam were numerically analyzed. Results show that the Airy beam drags particles …

In overall water splitting, oxygen evolution reaction is a rate-determining step due to its
complex reaction processes with sluggish kinetics, so design of bifunctional electrocatalyst with …