The DNA sequencing technologies in use today produce either highly accurate short reads
or less-accurate long reads. We report the optimization of circular consensus sequencing (…

10.7554/eLife.04580.001 Animals discriminate stimuli, learn their predictive value and use
this knowledge to modify their behavior. In Drosophila, the mushroom body (MB) plays a key …

The repetitive nature and complexity of some medically relevant genes poses a challenge
for their accurate analysis in a clinical setting. The Genome in a Bottle Consortium has …

Circular consensus sequencing with Pacific Biosciences (PacBio) technology generates
long (10–25 kilobases), accurate ‘HiFi’ reads by combining serial observations of a DNA …

Genome in a Bottle benchmarks are widely used to help validate clinical sequencing pipelines
and develop variant calling and sequencing methods. Here we use accurate linked and …

Infection results in the rapid activation of immunity genes in the Drosophila fat body. Two
classes of transcription factors have been implicated in this process: the REL-containing …

Tandem repeat (TR) variation is associated with gene expression changes and numerous
rare monogenic diseases. Although long-read sequencing provides accurate full-length …

Purpose This study aimed to provide comprehensive diagnostic and candidate analyses in
a pediatric rare disease cohort through the Genomic Answers for Kids program. Methods …

Background Long-read sequencing (LRS) techniques have been very successful in identifying
structural variants (SVs). However, the high error rate of LRS made the detection of small …

Similar to many insect species, Drosophila melanogaster is capable of maintaining a stable
flight trajectory for periods lasting up to several hours. Because aerodynamic torque is …