The principal function of CRISPR–Cas systems in archaea and bacteria is defence against
mobile genetic elements (MGEs), including viruses, plasmids and transposons. However, the …

The RNA-guided endonuclease Cas9 has emerged as a versatile genome-editing platform.
However, the size of the commonly used Cas9 from Streptococcus pyogenes (SpCas9) …

The RNA-guided endonuclease Cas9 is a versatile genome-editing tool with a broad range
of applications from therapeutics to functional annotation of genes. Cas9 creates double-…

Duchenne muscular dystrophy (DMD) is a devastating disease affecting about 1 out of 5000
male births and caused by mutations in the dystrophin gene. Genome editing has the …

Frame-disrupting mutations in the DMD gene, encoding dystrophin, compromise myofiber
integrity and drive muscle deterioration in Duchenne muscular dystrophy (DMD). Removing …

The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets with a
protospacer adjacent motif (PAM) and complementarity to the guide RNA. Recently, we …

The RNA-guided endonuclease Cpf1 is a promising tool for genome editing in eukaryotic
cells 1 , 2 , 3 , 4 , 5 , 6 , 7 . However, the utility of the commonly used Acidaminococcus sp. …

Bacterial class 2 CRISPR-Cas systems utilize a single RNA-guided protein effector to mitigate
viral infection. We aggregated genomic data from multiple sources and constructed an …

Type V CRISPR-Cas systems are distinguished by a single RNA-guided RuvC domain-containing
effector, Cas12. Although effectors of subtypes VA (Cas12a) and VB (Cas12b) have …

Precisely measuring the location and frequency of DNA double-strand breaks (DSBs) along
the genome is instrumental to understanding genomic fragility, but current methods are …