Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis
Recently developed DNA base editing methods enable the direct generation of desired point
mutations in genomic DNA without generating any double-strand breaks 1 , 2 – 3 , but the …
mutations in genomic DNA without generating any double-strand breaks 1 , 2 – 3 , but the …
Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice
S Ling, S Yang, X Hu, D Yin, Y Dai, X Qian… - Nature Biomedical …, 2021 - nature.com
Therapeutic genome editing requires effective and targeted delivery methods. The delivery
of Cas9 mRNA using adeno-associated viruses has led to potent in vivo therapeutic efficacy, …
of Cas9 mRNA using adeno-associated viruses has led to potent in vivo therapeutic efficacy, …
[HTML][HTML] Screened AAV variants permit efficient transduction access to supporting cells and hair cells
X Hu, J Wang, X Yao, Q Xiao, Y Xue, S Wang, L Shi… - Cell Discovery, 2019 - nature.com
Dear Editor, Genetic malady is a significant pathogenesis underlying sensorineural hearing
loss. More than half of the individuals with pre-lingual hearing loss are verified with inherited …
loss. More than half of the individuals with pre-lingual hearing loss are verified with inherited …
[PDF][PDF] Glia-to-neuron conversion by CRISPR-CasRx alleviates symptoms of neurological disease in mice
H Zhou, J Su, X Hu, C Zhou, H Li, Z Chen, Q Xiao… - Cell, 2020 - cell.com
Conversion of glial cells into functional neurons represents a potential therapeutic approach
for replenishing neuronal loss associated with neurodegenerative diseases and brain injury…
for replenishing neuronal loss associated with neurodegenerative diseases and brain injury…
[HTML][HTML] Homology-mediated end joining-based targeted integration using CRISPR/Cas9
X Yao, X Wang, X Hu, Z Liu, J Liu, H Zhou, X Shen… - Cell research, 2017 - nature.com
Targeted integration of transgenes can be achieved by strategies based on homologous
recombination (HR), microhomology-mediated end joining (MMEJ) or non-homologous end …
recombination (HR), microhomology-mediated end joining (MMEJ) or non-homologous end …
In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice
Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression of
multiple genes remains challenging. We generated a transgenic mouse using an improved …
multiple genes remains challenging. We generated a transgenic mouse using an improved …
[HTML][HTML] One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs
The CRISPR/Cas9 system is an efficient gene-editing method, but the majority of gene-edited
animals showed mosaicism, with editing occurring only in a portion of cells. Here we show …
animals showed mosaicism, with editing occurring only in a portion of cells. Here we show …
[HTML][HTML] CRISPR/Cas9-mediated targeted chromosome elimination
Background The CRISPR/Cas9 system has become an efficient gene editing method for
generating cells carrying precise gene mutations, including the rearrangement and deletion of …
generating cells carrying precise gene mutations, including the rearrangement and deletion of …
[PDF][PDF] Tild-CRISPR allows for efficient and precise gene knockin in mouse and human cells
X Yao, M Zhang, X Wang, W Ying, X Hu, P Dai… - Developmental cell, 2018 - cell.com
The targeting efficiency of knockin sequences via homologous recombination (HR) is
generally low. Here we describe a method we call Tild-CRISPR (targeted integration with …
generally low. Here we describe a method we call Tild-CRISPR (targeted integration with …
Synthesis of porous carbon-doped g-C3N4 nanosheets with enhanced visible-light photocatalytic activity
N Bao, X Hu, Q Zhang, X Miao, X Jie, S Zhou - Applied Surface Science, 2017 - Elsevier
The porous carbon-doped gC 3 N 4 nanosheets photocatalysts (NSs-APAM) were synthesized
using anionic polyacrylamide (APAM) as the intercalator and carbon source via the …
using anionic polyacrylamide (APAM) as the intercalator and carbon source via the …