The CRISPR-Cas system is a powerful tool for generating genetically modified animals;
however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here …

Although single-component Class 2 CRISPR systems, such as type II Cas9 or type V Cas12a
(Cpf1), are widely used for genome editing in eukaryotic cells, the application of multi-…

Photosynthesis is the primary source ofdry matter production and grain yield inrice. Study
on genetics of photosynthesisand related physiological characters isvery important to rice …

Background CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient
approaches to create loxP-flanked (floxed) alleles remain elusive. Results Here, we show that …

Background CRISPR-Cas9 gene-editing technology has facilitated the generation of knockout
mice, providing an alternative to cumbersome and time-consuming traditional embryonic …

… qSBR-3 may be due to the pleiotropic effect of qCL-3 (Kunihiro et al. 2002). Thus, since it was
… Oro and Y. Kido (1983) The syringe inoculation method for selecting rice plants resistant to …

TAL Effector Nucleases (TALENs) are versatile tools for targeted gene editing in various
species. However, their efficiency is still insufficient, especially in mammalian embryos. Here, …

Purpose To investigate the added value of lung perfused blood volume (LPBV) using dual-energy
CT for the evaluation of intrapulmonary clot (IPC) in patients suspected of having …

In this study, an indica variety Zhai Ye Qing 8 (ZYQ8) and a japonica variety Jing Xi 17 (JX17)
and their double haploid (DH) population were used. Sheath blight resistance of the DH …

Severe combined immunodeficiency (SCID) mice, the most widely used animal model of
DNA-PKcs (Prkdc) deficiency, have contributed enormously to our understanding of …