To study fidelity of RNA polymerase II (Pol II), we analyzed properties of the 6-azauracil-sensitive
and TFIIS-dependent E1103G mutant of rbp1 (rpo21), the gene encoding the catalytic …

… Jin Young Kang,1 Rachel Anne Mooney,2 Yuri Nedialkov,3,4 Jason Saba,2 Tatiana V. …
states are indicated on the y axis. Data shown are means and SD from at least three replicates. …

Rpb9 is a small non-essential subunit of yeast RNA polymerase II located on the surface on
the enzyme. Deletion of the RPB9 gene shows synthetic lethality with the low fidelity rpb1-…

Our laboratory has developed methods for transient state kinetic analysis of human RNA
polymerase II elongation. In these studies, multiple conformations of the RNA polymerase II …

Numerous classes of riboswitches have been found to regulate bacterial gene expression
in response to physiological cues, offering new paths to antibacterial drugs. As common …

We report a "running start, two-bond" protocol to analyze elongation by human RNA
polymerase II (RNAP II). In this procedure, the running start allowed us to measure rapid rates of …

We previously showed that injection of homogenous staphylococcal protein AV antigen fusion
peptide into mice delayed allograft rejection and suppressed the major proinflammatory …

10.7554/eLife.36349.001 RfaH, a transcription regulator of the universally conserved NusG/Spt5
family, utilizes a unique mode of recruitment to elongating RNA polymerase to activate …

RNA folding during transcription directs an order of folding that can determine RNA structure
and function. However, the experimental study of cotranscriptional RNA folding has been …

… raised against outer membranes of Y. pestis was enriched for anti-KatY. A sequenced ∼16-kb
Y. pestis DNA insert of a … Y. pestis and closely related enteropathogenic yersiniae (…