Ten years of next-generation sequencing technology

EL Van Dijk, H Auger, Y Jaszczyszyn, C Thermes - Trends in genetics, 2014 - cell.com
Ten years ago next-generation sequencing (NGS) technologies appeared on the market.
During the past decade, tremendous progress has been made in terms of speed, read length, …

The third revolution in sequencing technology

EL Van Dijk, Y Jaszczyszyn, D Naquin, C Thermes - Trends in Genetics, 2018 - cell.com
Forty years ago the advent of Sanger sequencing was revolutionary as it allowed complete
genome sequences to be deciphered for the first time. A second revolution came when next-…

Library preparation methods for next-generation sequencing: tone down the bias

EL Van Dijk, Y Jaszczyszyn, C Thermes - Experimental cell research, 2014 - Elsevier
Next-generation sequencing (NGS) has caused a revolution in biology. NGS requires the
preparation of libraries in which (fragments of) DNA or RNA molecules are fused with adapters …

[HTML][HTML] Replication landscape of the human genome

…, M Kahli, Y d'Aubenton-Carafa, Y Jaszczyszyn… - Nature …, 2016 - nature.com
Despite intense investigation, human replication origins and termini remain elusive. Existing
data have shown strong discrepancies. Here we sequenced highly purified Okazaki …

Genomics in the long-read sequencing era

EL van Dijk, D Naquin, K Gorrichon, Y Jaszczyszyn… - Trends in Genetics, 2023 - cell.com
Long-read sequencing (LRS) technologies have provided extremely powerful tools to explore
genomes. While in the early years these methods suffered technical limitations, they have …

[PDF][PDF] Dual roles of poly (dA: dT) tracts in replication initiation and fork collapse

…, A Canela, H Fu, C Redon, SC Pruitt, Y Jaszczyszyn… - Cell, 2018 - cell.com
Replication origins, fragile sites, and rDNA have been implicated as sources of chromosomal
instability. However, the defining genomic features of replication origins and fragile sites …

[HTML][HTML] Transcription-mediated organization of the replication initiation program across large genes sets common fragile sites genome-wide

…, M Schmidt, V Nähse, Y Jaszczyszyn… - Nature …, 2019 - nature.com
Common fragile sites (CFSs) are chromosome regions prone to breakage upon replication
stress known to drive chromosome rearrangements during oncogenesis. Most CFSs nest in …

Mistimed origin licensing and activation stabilize common fragile sites under tight DNA-replication checkpoint activation

…, M Schmidt, S El-Hilali, Y Jaszczyszyn… - Nature Structural & …, 2023 - nature.com
Genome integrity requires replication to be completed before chromosome segregation.
The DNA-replication checkpoint (DRC) contributes to this coordination by inhibiting CDK1, …

[HTML][HTML] Dynamics of the compartmentalized Streptomyces chromosome during metabolic differentiation

…, O Lespinet, P Leblond, Y Jaszczyszyn… - Nature …, 2021 - nature.com
Bacteria of the genus Streptomyces are prolific producers of specialized metabolites, including
antibiotics. The linear chromosome includes a central region harboring core genes, as …

[PDF][PDF] Paramecium Polycomb repressive complex 2 physically interacts with the small RNA-binding PIWI protein to repress transposable elements

…, A Michaud, I Cohen, A Humbert, Y Jaszczyszyn… - Developmental cell, 2022 - cell.com
Polycomb repressive complex 2 (PRC2) maintains transcriptionally silent genes in a
repressed state via deposition of histone H3K27-trimethyl (me3) marks. PRC2 has also been …