The comprehensive reconstruction of cell lineages in complex multicellular organisms is a
central goal of developmental biology. We present an open-source computational framework …

Imaging fast cellular dynamics across large specimens requires high resolution in all dimensions,
high imaging speeds, good physical coverage and low photo-damage. To meet these …

Optimal image quality in light-sheet microscopy requires a perfect overlap between the
illuminating light sheet and the focal plane of the detection objective. However, mismatches …

We present the Real-time Accurate Cell-shape Extractor (RACE), a high-throughput image
analysis framework for automated three-dimensional cell segmentation in large-scale images…

Light-sheet microscopy is a powerful method for imaging the development and function of
complex biological systems at high spatiotemporal resolution and over long time scales. Such …

The ability to visualize and quantitatively measure dynamic biological processes in vivo and
at high spatiotemporal resolution is of fundamental importance to experimental …

Animal survival requires a functioning nervous system to develop during embryogenesis.
Newborn neurons must assemble into circuits producing activity patterns capable of instructing …

Gastric cancer is the second leading cause of worldwide cancer mortality, yet the underlying
genomic alterations remain poorly understood. Here we perform exome and transcriptome …

Histone post-translational modifications are key gene expression regulators, but their rapid
dynamics during development remain difficult to capture. We applied a Fab-based live …

Clonal analysis is helping us understand the dynamics of cell replacement in homeostatic
adult tissues ( Simons and Clevers, 2011 ). Such an analysis, however, has not yet been …