The highly conserved protein γ-tubulin is required for microtubule nucleation in vivo. When
viewed in the electron microscope, a highly purified γtubulin complex from Xenopus …

Pericentrin and γ-tubulin are integral centrosome proteins that play a role in microtubule
nucleation and organization. In this study, we examined the relationship between these proteins …

The mipA gene of A. nidulans encodes a newly discovered member of the tubulin
superfamily of proteins, y-tubulin. In A. nidulans, y-tubulin is essential for nuclear division and …

Ran, a small guanosine triphosphatase, is suggested to have additional functions beyond its
well-characterized role in nuclear trafficking. Guanosine triphosphate–bound Ran, but not …

The activated form of Ran (Ran-GTP) stimulates spindle assembly in Xenopus laevis egg
extracts, presumably by releasing spindle assembly factors, such as TPX2 (target protein for …

γ-tubulin exists in two related complexes in Drosophila embryo extracts (Moritz, M., Y. Zheng,
BM Alberts, and K. Oegema. 1998. J. Cell Biol. 142:1– 12). Here, we report the purification …

The guanosine triphosphatase Ran stimulates assembly of microtubule asters and spindles
in mitoticXenopus egg extracts. A carboxyl-terminal region of the nuclear-mitotic apparatus …

Mitotic spindle morphogenesis is a series of highly coordinated movements that lead to
chromosome segregation and cytokinesis. We report that the intermediate filament protein lamin …

Microtubule nucleation from centrosomes involves a lockwasher-shaped protein complex
containing γ-tubulin, named the γ-tubulin ring complex (γTuRC). Here we investigate the …

Proper chromosome segregation requires the attachment of sister kinetochores to microtubules
from opposite spindle poles to form bi-oriented chromosomes on the metaphase spindle. …