The organization of a cell emerges from the interactions in protein networks. The interactome
is critically dependent on the strengths of interactions and the cellular abundances of the …

During mitosis, adherent animal cells undergo a drastic shape change, from essentially flat
to round 1 , 2 , 3 . Mitotic cell rounding is thought to facilitate organization within the mitotic …

The interpretation of genome sequences requires reliable and standardized methods to assess
protein function at high throughput. Here we describe a fast and reliable pipeline to study …

Chromosome segregation and cell division are essential, highly ordered processes that depend
on numerous protein complexes. Results from recent RNA interference screens indicate …

The centromere is the chromosomal site that joins to microtubules during mitosis for proper
segregation. Determining the location of a centromere-specific histone H3 called CENP-A at …

Centrosomes in animal cells are dynamic organelles with a proteinaceous matrix of
pericentriolar material assembled around a pair of centrioles. They organize the microtubule …

Defects in kinetochore proteins often lead to aneuploidy and cancer. Mis12–Mtw1 is a
conserved, essential kinetochore protein family. Here, we show that a Mis12 core complex exists …

The anaphase-promoting complex/cyclosome (APC/C) bound to CDC20 (APC/C CDC20 )
initiates anaphase by ubiquitylating B-type cyclins and securin. During chromosome bi-…

The cell cortex is a key structure for the regulation of cell shape and tissue organization. To
reach a better understanding of the mechanics and dynamics of the cortex, we study here …

During animal cell cytokinesis, the spindle directs contractile ring assembly by activating
RhoA in a narrow equatorial zone. Rapid GTPase activating protein (GAP)-mediated …