User profiles for Yutetsu Kuruma
Yutetsu KurumaEarth-Life Science Institute Verified email at elsi.jp Cited by 1972 |
[HTML][HTML] Artificial photosynthetic cell producing energy for protein synthesis
Attempts to construct an artificial cell have widened our understanding of living organisms.
Many intracellular systems have been reconstructed by assembling molecules, however the …
Many intracellular systems have been reconstructed by assembling molecules, however the …
[HTML][HTML] A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells
Synthetic biology is an emerging field that aims at constructing artificial biological systems
by combining engineering and molecular biology approaches. One of the most ambitious …
by combining engineering and molecular biology approaches. One of the most ambitious …
Compartmentalized reactions as a case of soft-matter biotechnology: synthesis of proteins and nucleic acids inside lipid vesicles
P Stano, P Carrara, Y Kuruma, TP de Souza… - Journal of Materials …, 2011 - pubs.rsc.org
In this mini-review we would like to summarize the recent advances in the field of protein
and nucleic acid synthesis inside lipid vesicles (liposomes). This research, which originated …
and nucleic acid synthesis inside lipid vesicles (liposomes). This research, which originated …
Cell‐free translation systems for protein engineering
Cell‐free translation systems have developed significantly over the last two decades and
improvements in yield have resulted in their use for protein production in the laboratory. These …
improvements in yield have resulted in their use for protein production in the laboratory. These …
Protein synthesis in liposomes with a minimal set of enzymes
In a significant step towards the construction of the semi-synthetic minimal cell, a protein
expression system with a minimal set of pure and specific enzymes is required. A novel cell-free …
expression system with a minimal set of pure and specific enzymes is required. A novel cell-free …
The PURE system for the cell-free synthesis of membrane proteins
Cell-free gene expression systems are biotechnological tools for the in vitro production of
proteins of interest. The addition of membrane vesicles (liposomes) enables the production of …
proteins of interest. The addition of membrane vesicles (liposomes) enables the production of …
Synthetic cells produce a quorum sensing chemical signal perceived by Pseudomonas aeruginosa
…, M Messina, A Zennaro, Y Kuruma… - Chemical …, 2018 - pubs.rsc.org
Recent developments in bottom-up synthetic biology (eg, lipid vesicle technology integrated
with cell-free protein expression systems) allow the generation of semi-synthetic minimal …
with cell-free protein expression systems) allow the generation of semi-synthetic minimal …
[HTML][HTML] Phospholipid synthesis inside phospholipid membrane vesicles
Construction of living artificial cells from genes and molecules can expand our understanding
of life system and establish a new aspect of bioengineering. However, growth and division …
of life system and establish a new aspect of bioengineering. However, growth and division …
In Vitro Synthesis of the E. coli Sec Translocon from DNA
Difficulties in constructing complex lipid/protein membranes have severely limited the
development of functional artificial cells endowed with vital membrane‐related functions. The Sec …
development of functional artificial cells endowed with vital membrane‐related functions. The Sec …
De Novo Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles
Cell division is the most dynamic event in the cell cycle. Recently, efforts have been made to
reconstruct it using the individual component proteins to obtain a better understanding of …
reconstruct it using the individual component proteins to obtain a better understanding of …