Enzyme-based chemical transformations typically proceed with high selectivity under mild
conditions, and are becoming increasingly important in the pharmaceutical and chemical …

The capacity to fix nitrogen is widely distributed in phyla of Bacteria and Archaea but has long
been considered to be absent from the Pseudomonas genus. We report here the complete …

Acidogenic and aciduric bacteria have developed several survival systems in various acidic
environments to prevent cell damage due to acid stress such as that on the human gastric …

The ability to engineer proteins by directed evolution requires functional expression of the
target polypeptide in a recombinant host suitable for construction and screening libraries of …

Background In recent years, it has been gradually realized that bacterial inclusion bodies (IBs)
could be biologically active. In particular, several proteins including green fluorescent …

Background Furfural and 5-hydroxymethylfurfural (HMF) are the degradation products of
lignocellulose during pretreatment operations and significantly inhibit the consequent …

The tumor microenvironment is a complex ecosystem formed by distinct and interacting cell
populations, and its composition is related to cancer prognosis and response to clinical …

Background Inactive protein inclusion bodies occur commonly in Escherichia coli (E. coli)
cells expressing heterologous proteins. Previously several independent groups have found …

The molecular chaperone GroEL from Escherichia coli is a member of the highly conserved
Hsp60 family of proteins that facilitates protein folding. A central question regarding the …

Background: Oxygenases catalyze the hydroxylation of a wide variety of organic substrates.
An ability to alter oxygenase substrate specificities and improve their activities and stabilities …