10.7554/eLife.34257.001 Single particle cryo-electron microscopy (cryoEM) is often performed
under the assumption that particles are not adsorbed to the air-water interfaces and in thin…

The insulin receptor is a dimeric protein that has a crucial role in controlling glucose
homeostasis, regulating lipid, protein and carbohydrate metabolism, and modulating brain …

Most protein particles prepared in vitreous ice for single-particle cryo-electron microscopy (cryo-EM)
are adsorbed to air–water or substrate–water interfaces, which can cause the …

The organization of genomic DNA into nucleosomes profoundly affects all DNA-related
processes in eukaryotes. The histone chaperone known as ‘facilitates chromatin transcription’ (…

SARS-CoV-2 cell entry is completed after viral spike (S) protein–mediated membrane fusion
between viral and host cell membranes. Stable prefusion and postfusion S structures have …

We present an update describing new features and applications of Spotiton, a novel
instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test …

Almost every aspect of cryo electron microscopy (cryoEM) has been automated over the last
few decades. One of the challenges that remains to be addressed is the robust and reliable …

According to the hierarchical identification principle, a hierarchical gradient based iterative
estimation algorithm is derived for multivariable output error moving average systems (ie, …

Fidaxomicin is an antibacterial drug in clinical use for treatment of Clostridium difficile
diarrhea. The active ingredient of fidaxomicin, lipiarmycin A3 (Lpm), functions by inhibiting …

The recently reported B.1.1.529 Omicron variant of severe acute respiratory syndrome
coronavirus-2 (SARS-CoV-2) includes 34 mutations in the spike protein relative to the Wuhan …