The eukaryotic CMG (Cdc45, Mcm2–7, GINS) helicase consists of the Mcm2–7 hexameric
ring along with five accessory factors. The Mcm2–7 heterohexamer, like other hexameric …

At the eukaryotic DNA replication fork, it is widely believed that the Cdc45–Mcm2–7–GINS (CMG)
helicase is positioned in front to unwind DNA and that DNA polymerases trail behind …

To initiate DNA replication, the origin recognition complex (ORC) and Cdc6 load an Mcm2–7
double hexamer onto DNA. Without ATP hydrolysis, ORC–Cdc6 recruits one Cdt1-bound …

The CMG helicase is composed of Cdc45, Mcm2–7 and GINS. Here we report the structure
of the Saccharomyces cerevisiae CMG, determined by cryo-EM at a resolution of 3.7–4.8 Å. …

Eukaryotic cells license each DNA replication origin during G1 phase by assembling a
prereplication complex that contains a Mcm2–7 (minichromosome maintenance proteins 2–7) …

The eukaryotic leading strand DNA polymerase (Pol) ε contains 4 subunits, Pol2, Dpb2, Dpb3
and Dpb4. Pol2 is a fusion of two B-family Pols; the N-terminal Pol module is catalytic and …

During replication initiation, the core component of the helicase—the Mcm2-7 hexamer—is
loaded on origin DNA as a double hexamer (DH). The two ring-shaped hexamers are …

The eukaryotic polymerase α (Pol α) synthesizes an RNA-DNA hybrid primer of 20–30
nucleotides. Pol α is composed of Pol1, Pol12, Primase 1 (Pri1), and Pri2. Pol1 and Pri1 contain …

DNA replication origins serve as sites of replicative helicase loading. In all eukaryotes, the
six-subunit origin recognition complex (Orc1-6; ORC) recognizes the replication origin. During …

High-resolution structures have not been reported for replicative helicases at a replication
fork at atomic resolution, a prerequisite to understanding the unwinding mechanism. The …