Long-read sequence assembly: a technical evaluation in barley

…, J Ens, H Gundlach, LB Boston, Z Tulpová… - The Plant …, 2021 - academic.oup.com
Sequence assembly of large and repeat-rich plant genomes has been challenging, requiring
substantial computational resources and often several complementary sequence assembly …

BioNano genome mapping of individual chromosomes supports physical mapping and sequence assembly in complex plant genomes

…, AR Hastie, S Chan, J Vrána, Z Tulpová… - Plant biotechnology …, 2016 - Wiley Online Library
The assembly of a reference genome sequence of bread wheat is challenging due to its
specific features such as the genome size of 17 Gbp, polyploid nature and prevalence of …

Prospects of telomere‐to‐telomere assembly in barley: Analysis of sequence gaps in the MorexV3 reference genome

P Navrátilová, H Toegelová, Z Tulpová… - Plant biotechnology …, 2022 - Wiley Online Library
The first gapless, telomere‐to‐telomere (T2T) sequence assemblies of plant chromosomes
were reported recently. However, sequence assemblies of most plant genomes remain …

[HTML][HTML] The dark matter of large cereal genomes: long tandem repeats

V Kapustová, Z Tulpová, H Toegelová, P Novák… - International journal of …, 2019 - mdpi.com
Reference genomes of important cereals, including barley, emmer wheat and bread wheat,
were released recently. Their comparison with genome size estimates obtained by flow …

Fine structure and transcription dynamics of bread wheat ribosomal DNA loci deciphered by a multi‐omics approach

Z Tulpová, A Kovařík, H Toegelová… - The Plant …, 2022 - Wiley Online Library
Three out of four RNA components of ribosomes are encoded by 45S ribosomal DNA (rDNA)
loci, which are organized as long head‐to‐tail tandem arrays of nearly identical units, …

Accessing a Russian wheat aphid resistance gene in bread wheat by long‐read technologies

Z Tulpová, H Toegelová, NLV Lapitan… - The plant …, 2019 - Wiley Online Library
Russian wheat aphid (RWA) (Diuraphis noxia Kurdjumov) is a serious invasive pest of small‐grain
cereals and many grass species. An efficient strategy to defy aphid attacks is to …

Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies

Z Tulpová, MC Luo, H Toegelová, P Visendi… - New biotechnology, 2019 - Elsevier
Bread wheat (Triticum aestivum L.) is a staple food for a significant part of the world’s
population. The growing demand on its production can be satisfied by improving yield and …

Chromosomal genomics facilitates fine mapping of a Russian wheat aphid resistance gene

…, J Batley, D Edwards, MC Luo, Z Tulpová… - Theoretical and Applied …, 2015 - Springer
Key message Making use of wheat chromosomal resources, we developed 11 gene-associated
markers for the region of interest, which allowed reducing gene interval and spanning it …

Flow Sorting–Assisted Optical Mapping

H Šimková, Z Tulpová, P Cápal - Plant Cytogenetics and Cytogenomics …, 2023 - Springer
Optical mapping—a technique that visualizes short sequence motives along DNA molecules
of hundred kilobases to megabase in size—has found an important place in genome …

Long-Read Structural and Epigenetic Profiling of a Kidney Tumor-matched Sample with Nanopore Sequencing and Optical Genome Mapping

S Margalit, Z Tulpová, T Detinis Zur, Y Michaeli, J Deek… - bioRxiv, 2024 - biorxiv.org
Carcinogenesis often involves significant alterations in the cancer genome architecture,
marked by large structural and copy number variations (SVs and CNVs) that are difficult to …