Progress in the Total Synthesis of the Gene for ala-tRNA
This extract was created in the absence of an abstract.
Excerpt
A major and continuing interest of this laboratory during the past fifteen years or so has been the development of the chemistry, especially the synthesis, of nucleic acids. Until the early sixties, our preoccupation was with methods for inter-nucleotide bond synthesis and with methods for suitable protection of different functional groups in nucleosides and nucleotides. Although organic chemical methods demand further investigation and refinement, nevertheless, during the past several years the available methods have enabled the synthesis of short chains of deoxyribopolynucleotides with known and controlled nucleotide sequences (Khorana et al., 1965). Indeed, when various deoxyribopolynucleotides with repeating patterns of nucleotide sequences were prepared and used as templates for the DNA polymerase of E. coli, it proved possible to prepare a corresponding series of double-stranded high molecular weight DNA-like polymers of defined sequences (Khorana et al., 1967). The availability of these DNA-like polymers in turn made possible precise studies of...
