A case for “StopGo”: Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go)
- John F. Atkins1,2,6,
- Norma M. Wills2,6,
- Gary Loughran1,6,
- Chih-Yu Wu3,
- Krishna Parsawar4,
- Martin D. Ryan5,
- Chung-Hsiung Wang3, and
- Chad C. Nelson4
- 1Biosciences Institute, University College Cork, Cork, Ireland
- 2Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112, USA
- 3Department of Entomology, National Taiwan University, Taipai, Republic of China
- 4Mass Spectrometry and Proteomics Core Facility, University of Utah, Salt Lake City, Utah 84112, USA
- 5Centre for Biomolecular Sciences, University of St. Andrews, St. Andrews KY16 9ST, United Kingdom
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↵6 These authors contributed equally to this work.
Abstract
When a eukaryotic mRNA sequence specifying an amino acid motif known as 2A is directly followed by a proline codon, two nonoverlapping proteins are synthesized. From earlier work, the second protein is known to start with this proline codon and is not created by proteolysis. Here we identify the C-terminal amino acid of an upstream 2A-encoded product from Perina nuda picorna-like virus that is glycine specified by the last codon of the 2A-encoding sequence. This is an example of recoding where 2A promotes unconventional termination after decoding of the glycine codon and continued translation beginning with the 3′ adjacent proline codon.
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Footnotes
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Reprint requests to: John F. Atkins, Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA; e-mail: john.atkins{at}genetics.utah.edu; fax: (801) 585 3910.
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Article published online ahead of print. Article and publication date are at http://www.rnajournal.org/cgi/doi/10.1261/rna.487907.
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- Received January 29, 2007.
- Accepted March 2, 2007.
- Copyright © 2007 RNA Society
