A Comprehensive Genetic Map of the Cattle Genome Based on 3802 Microsatellites

  1. Naoya Ihara1,
  2. Akiko Takasuga1,
  3. Kazunori Mizoshita2,
  4. Haruko Takeda1,3,
  5. Mayumi Sugimoto4,
  6. Yasushi Mizoguchi1,
  7. Takashi Hirano1,
  8. Tomohito Itoh1,5,
  9. Toshio Watanabe1,
  10. Kent M. Reed6,
  11. Warren M. Snelling7,
  12. Steven M. Kappes7,
  13. Craig W. Beattie8,
  14. Gary L. Bennett7, and
  15. Yoshikazu Sugimoto1,9
  1. 1Shirakawa Institute of Animal Genetics, Odakura, Nishigo, Nishi-shirakawa, Fukushima, 961-8061, Japan
  2. 2Cattle Breeding Development Institute Kagoshima, Kagoshima, 899-8212, Japan
  3. 3Department of Genetics, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium
  4. 4National Livestock Breeding Center, Odakura, Nishigo, Nishi-shirakawa, Fukushima, 961-8061, Japan
  5. 5Livestock Improvement Association of Japan, Inc., Maebashi, Gunma, 351-0121, Japan
  6. 6Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota 55108, USA
  7. 7U.S. Department of Agriculture (USDA), Agricultural Research Service (ARS), U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166, USA
  8. 8Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557, USA

Abstract

A microsatellite-based high-density genetic map facilitates for fine mapping of hereditary traits of interest, characterization of meiosis, and providing a foundation for physical map construction. Here, we developed a comprehensive genetic map on the basis of >880,000 genotypes across the USDA MARC cattle reference families with a potential genetic resolution of 0.8 cM at the 95% confidence level (∼800 kb in the bovine genome). We incorporated 2325 microsatellites into the second-generation genetic map by linkage analysis based on sex-averaged two-point LOD scores (>3.0), of which 2293 were fine-mapped by multipoint linkage analysis. The new 3160-cM map comprised of 29 sex-averaged autosomal linkage groups and a sex-specific X-chromosome linkage group includes 3960 markers with 2389 positions, resulting in an average interval size of 1.4 cM. More than half (51%) of the total length of the map is covered with intervals of 2.0 cM or less, and the largest gap is a 10.2-cM interval on the X-linkage group. The new map should accelerate fine mapping and positional cloning of genes for genetic diseases and economically important traits in cattle, as well as related livestock species, such as sheep and goat.

Footnotes

  • [Supplemental material is available online at www.genome.org. Marker information of new microsatellites is available from DDBJ under accession nos. AB164707 to AB166543 including flanking sequences and AB166544 to AB166659 for only primer sequences. Linkage groups for all autosomes and X- and Y-chromosomes are presented at http://www.marc.usda.gov/genome/genome.html.]

  • Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.2741704.

  • ↵9 Corresponding author. E-MAIL kazusugi{at}siag.or.jp; FAX 81-248-25-5725.

    • Accepted August 11, 2004.
    • Received April 2, 2004.
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  1. doi: 10.1101/gr.2741704 Genome Res. 14: 1987-1998 Cold Spring Harbor Laboratory Press

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