Natural variation in genome architecture among 205 Drosophila melanogaster Genetic Reference Panel lines
- Wen Huang1,10,
- Andreas Massouras2,3,10,
- Yutaka Inoue4,
- Jason Peiffer1,
- Miquel Ràmia5,
- Aaron M. Tarone6,
- Lavanya Turlapati1,
- Thomas Zichner7,
- Dianhui Zhu8,12,
- Richard F. Lyman1,
- Michael M. Magwire1,13,
- Kerstin Blankenburg8,
- Mary Anna Carbone1,
- Kyle Chang8,
- Lisa L. Ellis6,
- Sonia Fernandez8,
- Yi Han8,
- Gareth Highnam9,
- Carl E. Hjelmen6,
- John R. Jack1,
- Mehwish Javaid8,
- Joy Jayaseelan8,
- Divya Kalra8,
- Sandy Lee8,
- Lora Lewis8,
- Mala Munidasa8,
- Fiona Ongeri8,
- Shohba Patel8,
- Lora Perales8,
- Agapito Perez8,
- LingLing Pu8,
- Stephanie M. Rollmann1,14,
- Robert Ruth8,
- Nehad Saada8,
- Crystal Warner8,15,
- Aneisa Williams8,
- Yuan-Qing Wu8,
- Akihiko Yamamoto1,
- Yiqing Zhang8,
- Yiming Zhu8,
- Robert R.H. Anholt1,
- Jan O. Korbel7,
- David Mittelman9,
- Donna M. Muzny8,
- Richard A. Gibbs8,
- Antonio Barbadilla5,11,
- J. Spencer Johnston6,11,
- Eric A. Stone1,11,
- Stephen Richards8,11,
- Bart Deplancke2,3,11 and
- Trudy F.C. Mackay1,11,16
- 1Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina 27595, USA;
- 2Laboratory of Systems Biology and Genetics, Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland;
- 3Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland;
- 4Center for Education in Liberal Arts and Sciences, Osaka University, Osaka-fu, 560-0043 Japan;
- 5Genomics, Bioinformatics and Evolution Group, Institut de Biotecnologia i de Biomedicina (IBB), Department of Genetics and Microbiology, Campus Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain;
- 6Department of Entomology, Texas A&M University, College Station, Texas 77843, USA;
- 7Genome Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany;
- 8Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas 77030 USA;
- 9Virginia Tech Virginia Bioinformatics Institute and Department of Biological Sciences, Virginia Tech, Blacksburg, Virginia 24061, USA
Abstract
The Drosophila melanogaster Genetic Reference Panel (DGRP) is a community resource of 205 sequenced inbred lines, derived to improve our understanding of the effects of naturally occurring genetic variation on molecular and organismal phenotypes. We used an integrated genotyping strategy to identify 4,853,802 single nucleotide polymorphisms (SNPs) and 1,296,080 non-SNP variants. Our molecular population genomic analyses show higher deletion than insertion mutation rates and stronger purifying selection on deletions. Weaker selection on insertions than deletions is consistent with our observed distribution of genome size determined by flow cytometry, which is skewed toward larger genomes. Insertion/deletion and single nucleotide polymorphisms are positively correlated with each other and with local recombination, suggesting that their nonrandom distributions are due to hitchhiking and background selection. Our cytogenetic analysis identified 16 polymorphic inversions in the DGRP. Common inverted and standard karyotypes are genetically divergent and account for most of the variation in relatedness among the DGRP lines. Intriguingly, variation in genome size and many quantitative traits are significantly associated with inversions. Approximately 50% of the DGRP lines are infected with Wolbachia, and four lines have germline insertions of Wolbachia sequences, but effects of Wolbachia infection on quantitative traits are rarely significant. The DGRP complements ongoing efforts to functionally annotate the Drosophila genome. Indeed, 15% of all D. melanogaster genes segregate for potentially damaged proteins in the DGRP, and genome-wide analyses of quantitative traits identify novel candidate genes. The DGRP lines, sequence data, genotypes, quality scores, phenotypes, and analysis and visualization tools are publicly available.
Footnotes
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↵10 Joint first authors
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↵11 Senior authors
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↵16 Corresponding author
E-mail trudy_mackay{at}ncsu.edu
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[Supplemental material is available for this article.]
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Article published online before print. Article, supplemental material, and publication date are at http://www.genome.org/cgi/doi/10.1101/gr.171546.113.
Freely available online through the Genome Research Open Access option.
- Received December 20, 2013.
- Accepted April 1, 2014.
This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0.