Lsh, a member of the SNF2 family, is required for genome-wide methylation
Abstract
Methylation patterns of the mammalian genome are thought to be crucial for development. The precise mechanisms designating specific genomic loci for methylation are not known. Targeted deletion ofLsh results in perinatal lethality with a rather normal development. We report here, however, that Lsh −/−mice show substantial loss of methylation throughout the genome. The hypomethylated loci comprise repetitive elements and single copy genes. This suggests that global genomic methylation is not absolutely required for normal embryogenesis. Based on the similarity of Lsh to other SNF2 chromatin remodeling proteins, it suggests that alteration of chromatin affects global methylation patterns in mice.
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Footnotes
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↵1 Corresponding author.
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E-MAIL muegge{at}mail.ncifcrf.gov; FAX (301) 846-7077.
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Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.929101.
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- Received July 18, 2001.
- Accepted September 26, 2001.
- Cold Spring Harbor Laboratory Press