The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS-mediated embryo patterning
Abstract
Developmental responses to the plant hormone auxin are thought to be mediated by interacting pairs from two protein families: short-lived inhibitory IAA proteins and ARF transcription factors binding to auxin-response elements. monopteros mutants lacking activating ARF5 and the auxin-insensitive mutant bodenlos fail to initiate the root meristem during early embryogenesis. Here we show that thebodenlos phenotype results from an amino-acid exchange in the conserved degradation domain of IAA12. BODENLOS and MONOPTEROS interact in the yeast two-hybrid assay and the two genes are coexpressed in early embryogenesis, suggesting that BODENLOS inhibits MONOPTEROS action in root meristem initiation.
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Footnotes
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↵1 Present address: Institut für Biologie III, Universität Freiburg, Germany
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↵2 Corresponding author.
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E-MAIL gerd.juergens{at}zmbp.uni-tuebingen.de; FAX 49-7071-295797.
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Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.229402.
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- Received March 5, 2002.
- Accepted May 2, 2002.
- Cold Spring Harbor Laboratory Press
