phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development

Nathan D. Lawson; Joshua W. Mugford; Brigid A. Diamond; Brant M. Weinstein

doi:10.1101/gad.1072203

phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development

Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA

Abstract

In this study, we utilize transgenic zebrafish with fluorescently labeled blood vessels to identify and characterize a mutant (y10) that displays specific defects in the formation of arteries, but not veins. We find that y10 encodes phospholipase C gamma-1 (plcg1), a known effector of receptor tyrosine kinase signaling. We further show that plcg1^y10 mutant embryos fail to respond to exogenous Vegf. Our results indicate that Plcg1 functions specifically downstream of the Vegf receptor during embryonic development to govern formation of the arterial system.

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Footnotes

↵1 Present address: Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605.
Corresponding author.
↵2 E-MAIL Nathan.Lawson{at}umassmed.edu; FAX (508) 856-5460.
Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.1072203.
- Accepted April 14, 2003.
- Received January 3, 2003.
Cold Spring Harbor Laboratory Press