The active spatial organization of the β-globin locus requires the transcription factor EKLF
Abstract
Three-dimensional organization of a gene locus is important for its regulation, as recently demonstrated for the β-globin locus. When actively expressed, the cis-regulatory elements of the β-globin locus are in proximity in the nuclear space, forming a compartment termed the Active Chromatin Hub (ACH). However, it is unknown which proteins are involved in ACH formation. Here, we show that EKLF, an erythroid transcription factor required for adult β-globin gene transcription, is also required for ACH formation. We conclude that transcription factors can play an essential role in the three-dimensional organization of gene loci.
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Footnotes
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Supplemental material is available at http://www.genesdev.org.
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Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.317004.
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↵1 These authors contributed equally to this work.
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↵2 Corresponding author. E-MAIL j.philipsen{at}erasmusmc.nl; FAX 31-10-4089468.
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- Accepted August 16, 2004.
- Received November 18, 2003.
- Cold Spring Harbor Laboratory Press