Nucleolar localization of early tRNA processing
- 1Institut de Genetique Moleculaire de Montpellier-Centre National de la Recherche Scientifique (CNRS), 34033 Montpellier Cedex 01, France; 2Department of Biological Chemistry and Program in Cellular and Molecular Biology, The University of Michigan, Ann Arbor, Michigan 48109-0606 USA; 3Department of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, New York, New York 10461 USA
Abstract
There is little information as to the location of early tRNA biosynthesis. Using fluorescent in situ hybridization in the budding yeast, Saccharomyces cerevisiae, examples of nuclear pre-tRNAs are shown to reside primarily in the nucleoli. We also probed the RNA subunit of RNase P. The majority of the signal from RNase P probes was nucleolar, with less intense signals in the nucleoplasm. These results demonstrate that a major portion of the tRNA processing pathway is compartmentalized in nucleoli with rRNA synthesis and ribosomal assembly. The spatial juxtaposition suggests the possibility of direct coordination between tRNA and ribosome biosynthesis.
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Footnotes
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↵4 Corresponding author.
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E-MAIL engelke{at}umich.edu; FAX (734) 763-7799.
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- Received May 21, 1998.
- Accepted June 23, 1998.
- Cold Spring Harbor Laboratory Press
