Nitric oxide coordinates metabolism, growth, and development via the nuclear receptor E75
- Lucía Cáceres1,3,
- Aleksandar S. Necakov1,3,4,
- Carol Schwartz1,
- Sandra Kimber2,
- Ian J.H. Roberts2 and
- Henry M. Krause1,5
- 1Banting and Best Department of Medical Research, Department of Molecular Biology, University of Toronto, Toronto, Ontario M5S 3E1, Canada;
- 2Department of Biology, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom
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↵3 These authors contributed equally to this work.
Abstract
Nitric oxide gas acts as a short-range signaling molecule in a vast array of important physiological processes, many of which include major changes in gene expression. How these genomic responses are induced, however, is poorly understood. Here, using genetic and chemical manipulations, we show that nitric oxide is produced in the Drosophila prothoracic gland, where it acts via the nuclear receptor ecdysone-induced protein 75 (E75), reversing its ability to interfere with its heterodimer partner, Drosophila hormone receptor 3 (DHR3). Manipulation of these interactions leads to gross alterations in feeding behavior, fat deposition, and developmental timing. These neuroendocrine interactions and consequences appear to be conserved in vertebrates.
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Footnotes
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↵5 Corresponding author.
E-mail h.krause{at}utoronto.ca.
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Supplemental material is available for this article.
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Article published online ahead of print. Article and publication date are online at http://www.genesdev.org/cgi/doi/10.1101/gad.2064111.
- Received April 27, 2011.
- Accepted June 1, 2011.
- Copyright © 2011 by Cold Spring Harbor Laboratory Press
Freely available online through the Genes & Development Open Access option.