Arabidopsis cryptochrome 1 interacts with SPA1 to suppress COP1 activity in response to blue light
- Bin Liu1,2,4,
- Zecheng Zuo2,3,4,
- Hongtao Liu2,
- Xuanming Liu3 and
- Chentao Lin2,5
- 1Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
- 2Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles, Los Angeles, California 90095, USA;
- 3College of Life Sciences, Hunan University, Changsha 410082, China
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↵4 These authors contributed equally to this work.
Abstract
Plant photoreceptors mediate light suppression of the E3 ubiquitin ligase COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1) to affect gene expression and photomorphogenesis. However, how photoreceptors mediate light regulation of COP1 activity remains unknown. We report here that Arabidopsis blue-light receptor cryptochrome 1 (CRY1) undergoes blue-light-dependent interaction with the COP1-interacting protein SPA1 (SUPPRESSOR OF PHYTOCHROME A). We further show that the CRY1–SPA1 interaction suppresses the SPA1–COP1 interaction and COP1-dependent degradation of the transcription factor HY5. These results are consistent with a hypothesis that photoexcited CRY1 interacts with SPA1 to modulate COP1 activity and plant development.
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Footnotes
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↵5 Corresponding author.
E-MAIL clin{at}mcdb.ucla.edu; FAX (310) 206-3987.
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Article published online ahead of print. Article and publication date are online at http://www.genesdev.org/cgi/doi/10.1101/gad.2025011.
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Supplemental material is available for this article.
- Received December 22, 2010.
- Accepted March 23, 2011.
- Copyright © 2011 by Cold Spring Harbor Laboratory Press
