The E3 ubiquitin ligase activity of RING1B is not essential for early mouse development
- Robert S. Illingworth1,
- Michael Moffat1,
- Abigail R. Mann,
- David Read,
- Chris J. Hunter,
- Madapura M. Pradeepa,
- Ian R. Adams and
- Wendy A. Bickmore
- Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH42XU, United Kingdom
- Corresponding authors: wendy.bickmore{at}igmm.ed.ac.uk, ian.adams{at}igmm.ed.ac.uk
-
↵1 These authors contributed equally to this work.
Abstract
Polycomb-repressive complex 1 (PRC1) and PRC2 maintain repression at many developmental genes in mouse embryonic stem cells and are required for early development. However, it is still unclear how they are targeted and how they function. We show that the ability of RING1B, a core component of PRC1, to ubiquitinate histone H2A is dispensable for early mouse embryonic development and much of the gene repression activity of PRC1. Our data support a model in which PRC1 and PRC2 reinforce each other's binding but suggest that the key functions of PRC1 lie beyond the enzymatic capabilities of RING1B.
Keywords
Footnotes
-
Supplemental material is available for this article.
-
Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.268151.115.
-
Freely available online through the Genes & Development Open Access option.
- Received June 30, 2015.
- Accepted August 20, 2015.
This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
