A majority of m⁶A residues are in the last exons, allowing the potential for 3′ UTR regulation

Abstract

We adapted UV CLIP (cross-linking immunoprecipitation) to accurately locate tens of thousands of m⁶A residues in mammalian mRNA with single-nucleotide resolution. More than 70% of these residues are present in the 3′-most (last) exons, with a very sharp rise (sixfold) within 150–400 nucleotides of the start of the last exon. Two-thirds of last exon m⁶A and >40% of all m⁶A in mRNA are present in 3′ untranslated regions (UTRs); contrary to earlier suggestions, there is no preference for location of m⁶A sites around stop codons. Moreover, m⁶A is significantly higher in noncoding last exons than in next-to-last exons harboring stop codons. We found that m⁶A density peaks early in the 3′ UTR and that, among transcripts with alternative polyA (APA) usage in both the brain and the liver, brain transcripts preferentially use distal polyA sites, as reported, and also show higher proximal m⁶A density in the last exons. Furthermore, when we reduced m6A methylation by knocking down components of the methylase complex and then examined 661 transcripts with proximal m6A peaks in last exons, we identified a set of 111 transcripts with altered (approximately two-thirds increased proximal) APA use. Taken together, these observations suggest a role of m⁶A modification in regulating proximal alternative polyA choice.