Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A
- 1Cancer Institute of the Japanese Foundation for Cancer Research (JFCR), Tokyo 135-8550, Japan;
- 2Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8112, Japan
- Corresponding author: thirota{at}jfcr.or.jp
Abstract
The chromokinesin KIF4A has been implicated in shaping mitotic chromosomes, but its functional relationship to condensin complexes remains controversial. Here, we found that, in mitosis, KIF4A associates with condensin I but not with condensin II. Mutational analyses indicated that the enrichment of condensin I to chromosomal axes depends on its association with KIF4A in a way that likely involves its motor activity. Remarkably, this interaction is required for condensin I to confer physiological properties to chromosomes. These observations provide an insight into how condensin I is enriched at chromosomal axes and underscore the significance of axial structure in organizing mitotic chromosomes.
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Supplemental material is available for this article.
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Article published online ahead of print. Article and publication date are online at http://www.genesdev.org/cgi/doi/10.1101/gad.282855.116.
- Received April 19, 2016.
- Accepted August 8, 2016.
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