Ligand-induced endocytosis of the pattern recognition receptor FLS2 in Arabidopsis

  1. Silke Robatzek1,2,
  2. Delphine Chinchilla, and
  3. Thomas Boller
  1. Zurich-Basel Plant Science Center, Botanical Institute, University of Basel, 4056 Basel, Switzerland

Abstract

Pattern-recognition receptors (PRRs) trigger innate immune responses in animals and plants. One such PRR is the flagellin receptor FLS2 in Arabidopsis. Here, we demonstrate that a functional fusion of FLS2 to the green fluorescent protein (GFP) resides in cell membranes of most tissues. Stimulation with the flagellin epitope flg22 induces its transfer into intracellular mobile vesicles, followed by degradation. FLS2 internalization depends on cytoskeleton and proteasome functions, and receptor activation. A variant FLS2 mutated in Thr 867, a potential phosphorylation site, binds flg22 normally, but is impaired in flg22 responses and FLS2 endocytosis. We propose that plant cells regulate pathogen-associated molecular pattern (PAMP)-mediated PRR activities by subcellular compartmentalization.

Keywords

Footnotes

  • Supplemental material is available at http://www.genesdev.org.

  • Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.366506.

  • 1 Present address: Max-Planck-Institut für Züchtungsforschung, Carlvon-Linné-Weg 10, 50829 Köln, Germany.

  • 2 Corresponding author. E-MAIL robatzek{at}mpiz-koeln.mpg.de; FAX 49-221-5062-353.

    • Accepted January 11, 2006.
    • Received September 19, 2005.
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