Culturing the Moss Physcomitrella patens
- David J. Cove,
- Pierre-François Perroud,
- Audra J. Charron,
- Stuart F. McDaniel,
- Abha Khandelwal and
- Ralph S. Quatrano1
- ↵1Corresponding author (rsq{at}wustl.edu)
INTRODUCTION
This article includes a series of methods for culturing the moss Physcomitrella patens at all stages of its life cycle. Gametophytes are axenically cultured on solid agar-based media and in shaken liquid cultures. For long-term storage of gametophytes, cultures are maintained on solid medium at 10°C in a very short day. Cryopreservation may also be used. Finally, sporophytes are generated by self-fertilization and sexual crossing.
