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Is Actin Filament Sliding Responsible for Endoplasmic Reticulum and Golgi Movement?

View ORCID ProfileJoseph F McKenna, View ORCID ProfileStephen E D Webb, View ORCID ProfileVerena Kriechbaumer, View ORCID ProfileChris Hawes
doi: https://doi.org/10.1101/347187
Joseph F McKenna
Oxford Brookes University;
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Stephen E D Webb
UKRI, BBSRC
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Verena Kriechbaumer
Oxford Brookes University;
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Chris Hawes
Oxford Brookes University;
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  • For correspondence: chawes@brookes.ac.uk
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Abstract

In plants both the Golgi apparatus and the endoplasmic reticulum (ER) are highly motile. The Golgi apparatus, consisting of numerous Golgi stacks, is physically tethered to the surface of the motile ER membrane. Evidence is inconclusive as to whether there is a direct interaction between these organelles and the actin cytoskeleton, although there are reports of linker proteins between actin filaments and the ER membrane. Here, we use a combination of fluorescence recovery after photobleaching and a photoactivation strategy to investigate whether myosin driven actin filament sliding is a contributing factor in ER movement and thus Golgi motility. Utilising three different actin binding fluorescent protein constructs we show that recovery of fluorescence after photobleaching and loss of fluorescence after photoactivation is impeded by overexpression of a truncated myosin tail domain known to slow ER movement. We conclude that ER movement is in part mediated by myosin driven sliding of actin filaments within bundles linked to the ER membrane.

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Posted June 14, 2018.
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Is Actin Filament Sliding Responsible for Endoplasmic Reticulum and Golgi Movement?
Joseph F McKenna, Stephen E D Webb, Verena Kriechbaumer, Chris Hawes
bioRxiv 347187; doi: https://doi.org/10.1101/347187
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Is Actin Filament Sliding Responsible for Endoplasmic Reticulum and Golgi Movement?
Joseph F McKenna, Stephen E D Webb, Verena Kriechbaumer, Chris Hawes
bioRxiv 347187; doi: https://doi.org/10.1101/347187

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