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Loss of a conserved MAPK causes catastrophic failure in assembly of a specialized cilium-like structure in Toxoplasma gondii

William J. O’Shaughnessy, Xiaoyu Hu, Tsebaot Beraki, Matthew McDougal, View ORCID ProfileMichael L. Reese
doi: https://doi.org/10.1101/2020.02.02.931022
William J. O’Shaughnessy
†Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX 75390 USA
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Xiaoyu Hu
†Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX 75390 USA
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Tsebaot Beraki
†Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX 75390 USA
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Matthew McDougal
‡Department of Microbiology, UT Southwestern Medical Center, Dallas, TX 75390 USA
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Michael L. Reese
†Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX 75390 USA
§Department of Biochemistry, UT Southwestern Medical Center, Dallas, TX 75390 USA
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  • ORCID record for Michael L. Reese
  • For correspondence: Michael.Reese@UTSouthwestern.edu
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Abstract

Primary cilia are important organizing centers that control diverse cellular processes. Apicomplexan parasites like Toxoplasma gondii have a specialized cilium-like structure called the conoid that organizes the secretory and invasion machinery critical for the parasites’ lifestyle. The proteins that initiate the biogenesis of this structure are largely unknown. We identified the Toxoplasma ortholog of the conserved kinase ERK7 as essential to conoid assembly. Parasites in which ERK7 has been depleted lose their conoids late during maturation and are immotile and thus unable to invade new host cells. This is the most severe phenotype to conoid biogenesis yet reported, and is made more striking by the fact that ERK7 is not a conoid protein, as it localizes just basal to the structure. ERK7 has been recently implicated in ciliogenesis in metazoan cells, and our data suggest that this kinase has an ancient and central role in regulating ciliogenesis throughout Eukaryota.

Footnotes

  • TOC: Toxoplasma gondii that lack the kinase ERK7 cannot invade or egress from their host cells, thereby blocking their replicative cycle. These defects are due to the loss of a specialized cilium-like structure called the conoid. Strikingly, the ultrastructural changes are specific to the conoid, and suggest an important role for ERK7 in its biogenesis.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted February 02, 2020.
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Loss of a conserved MAPK causes catastrophic failure in assembly of a specialized cilium-like structure in Toxoplasma gondii
William J. O’Shaughnessy, Xiaoyu Hu, Tsebaot Beraki, Matthew McDougal, Michael L. Reese
bioRxiv 2020.02.02.931022; doi: https://doi.org/10.1101/2020.02.02.931022
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Loss of a conserved MAPK causes catastrophic failure in assembly of a specialized cilium-like structure in Toxoplasma gondii
William J. O’Shaughnessy, Xiaoyu Hu, Tsebaot Beraki, Matthew McDougal, Michael L. Reese
bioRxiv 2020.02.02.931022; doi: https://doi.org/10.1101/2020.02.02.931022

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